Anticatabolic Effects of Morin through the Counteraction of Interleukin-1β-Induced Inflammation in Rat Primary Chondrocytes

Morin, a flavonoid isolated from various medicinal herbal plants, has an anti-inflammatory effect. This study aimed to elucidate the anticatabolic effects and cellular mechanism of morin against interleukin-1 beta (IL-1 beta) in rat primary chondrocytes. Morin at 10-100 mu M did not affect the viability of rat primary chondrocytes. Treatment with morin for 21 days ameliorated the IL-1 beta-induced decrease in extracellular matrix. Furthermore, treatment with morin attenuated IL-1 beta-induced proteoglycan loss in the articular cartilage through suppression of catabolic factors, such as matrix metalloproteinases, inflammatory mediators, and pro-inflammatory cytokines. These data indicated that morin exerted anticatabolic effects that can prevent and reduce progressive degeneration of the articular cartilage, and thus may be a potential candidate treatment for osteoarthritis.