Docosahexaenoic acid prevented tumor necrosis factor alpha-induced endothelial dysfunction and senescence

被引：30
|
作者：
Yamagata, Kazuo [1 ]
Suzuki, Sayaka [1 ]
Tagami, Motoki [2 ]
机构：
[1] Nihon Univ NUBS, Coll Bioresource Sci, Dept Food Biosci & Biotechnol, Fujisawa, Kanagawa, Japan
[2] Sanraku Hosp, Dept Internal Med, Chiyoda Ku, Tokyo, Japan
来源：
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS | 2016年 / 104卷
关键词：
Endothelial cells; Dysfunction; Senescence; DHA; TNF alpha; DENSITY-LIPOPROTEIN RECEPTOR-1; TNF-ALPHA; OXIDATIVE STRESS; CELL-ADHESION; GROWTH-FACTOR; LIFE-SPAN; EXPRESSION; P66(SHC); GENE; PHENOTYPE;
D O I：
10.1016/j.plefa.2015.10.006
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We investigated how docosahexaenoic acid (DHA) regulated tumor necrosis factor-alpha (TNF-alpha)-induced senescence and dysfunction in endothelial cells (EC). We used RT-PCR to examine the expression of several genes related to senescence and dysfunction in EC. TNF-alpha-induced p21 protein levels were investigated by Western blot (WB) and fluorescence antibody techniques. TNF-alpha induced the senescence marker P-galactosidase and the expression of several senescence and endothelial dysfunction-related genes, e.g., CDKN1A, SHC1 and GLB1. DHA attenuated TNF-alpha-induced senescence-related gene expression and p21 protein expression. DHA attenuated TNF-alpha-induced gene expression related to dysfunction of EC, such as plasminogen activator inhibitor 1 (SERPINE1), lectin-like oxidized low-density lipoprotein receptor-1 (OLR1), thromboxane A2 receptor (TXA2R) and p38 MAPK (MAPK14). DHA reversed the TNF-alpha-mediated reduction of endothelial nitric oxide synthase (NOS3) gene expression. TNF-alpha-mediated upregulation of these genes was inhibited by allopurinol and apocynin. These results indicated that DHA regulated the expression of several genes that are associated with senescence and dysfunction of EC. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：11 / 18
页数：8
相关论文
共 50 条
  • [21] A20 contributes to tumor necrosis factor alpha-induced tolerance in the mouse liver.
    Sass, G
    Shembade, ND
    Tiegs, G
    HEPATOLOGY, 2004, 40 (04) : 284A - 284A
  • [22] Upregulation of Tumor Necrosis Factor Alpha-Induced Protein 3 mRNA in Mild Psoriasis Vulgaris
    Zhang, Xia
    Xia, Ping
    Zhang, Li
    Zhang, Zhenghua
    CLINICAL AND VACCINE IMMUNOLOGY, 2013, 20 (08) : 1341 - 1341
  • [23] Regulatory roles of tumor necrosis factor alpha-induced proteins (TNFAIPs) 3 and 9 in arthritis
    Matsumoto, Isao
    Inoue, Asuka
    Takai, Chinatsu
    Umeda, Naoto
    Tanaka, Yuki
    Kurashima, Yuko
    Sumida, Takayuki
    CLINICAL IMMUNOLOGY, 2014, 153 (01) : 73 - 78
  • [24] Mutation analysis of tumor necrosis factor alpha-induced protein 3 gene in Hodgkin lymphoma
    Etzel, Barbara-Magdalena
    Gerth, Melanie
    Chen, Yuan
    Wuensche, Elisa
    Facklam, Tina
    Beck, James F.
    Guntinas-Lichius, Orlando
    Petersen, Iver
    PATHOLOGY RESEARCH AND PRACTICE, 2017, 213 (03) : 256 - 260
  • [25] Selenium Reduces Early Signs of Tumor Necrosis Factor Alpha-Induced Meniscal Tissue Degradation
    Haefelein, Klaus
    Preusse-Prange, Andrea
    Behrendt, Peter
    Kurz, Bodo
    BIOLOGICAL TRACE ELEMENT RESEARCH, 2017, 177 (01) : 80 - 89
  • [26] Tumor necrosis factor alpha-induced protein 3 diversity influence nasopharyngeal carcinoma risk
    Arij, B. C.
    Hayet, D.
    Abderraouf, G.
    Wahid, B.
    Tasnim, M.
    Latifa, H.
    Ghalia, K.
    Jihen, B.
    Narjes, C.
    Catherine, F.
    Rajagopal, K.
    Charron, D.
    Fethi, G.
    Ryad, T.
    TISSUE ANTIGENS, 2011, 77 (05): : 511 - 511
  • [27] Tumor necrosis factor alpha-induced vascular leakage involves PECAM1 phosphorylation
    Ferrero, E
    Villa, A
    Ferrero, ME
    Toninelli, E
    Bender, JR
    Pardi, R
    Zocchi, MR
    CANCER RESEARCH, 1996, 56 (14) : 3211 - 3215
  • [28] THERMAL ENHANCEMENT OF BOTH TUMOR-NECROSIS-FACTOR ALPHA-INDUCED SYSTEMIC TOXICITY AND TUMOR CURE IN RATS
    VANDERZEE, J
    VANDENAARDWEG, GJMJ
    VANRHOON, GC
    VANDENBERG, AP
    DEWIT, R
    BRITISH JOURNAL OF CANCER, 1995, 71 (06) : 1158 - 1162
  • [29] Protective effect of photodegradation product of nifedipine against tumor necrosis factor alpha-induced oxidative stress in human glomerular endothelial cells
    Fukuhara, Yayoi
    Tsuchiya, Koichiro
    Horinouchi, Yuya
    Tajima, Soichiro
    Kihira, Yoshitaka
    Hamano, Shuichi
    Kawazoe, Kazuyoshi
    Ikeda, Yasumasa
    Ishizawa, Keisuke
    Tomita, Shuhei
    Tamaki, Toshiaki
    JOURNAL OF MEDICAL INVESTIGATION, 2011, 58 (1-2): : 118 - 126
  • [30] Sympathetic modulation of tumor necrosis factor alpha-induced nociception in the presence of oral squamous cell carcinoma
    Atherton, Megan A.
    Park, Stella
    Horan, Nicole L.
    Nicholson, Samuel
    Dolan, John C.
    Schmidt, Brian L.
    Scheff, Nicole N.
    PAIN, 2023, 164 (01) : 27 - 42
12345