Preservation of organic matter and alteration of its carbon and nitrogen isotope composition during simulated and in situ early sedimentary diagenesis

The carbon and nitrogen isotope composition of organic matter has been widely used to trace biogeochemical processes in marine and lacustrine environments. In order to reconstruct past environmental changes from sedimentary organic matter, it is crucial to consider potential alteration of the primary isotopic signal by bacterial degradation in the water column and during early diagenesis in the sediments. In a series of oxic and anoxic incubation experiments, we examined the fate of organic matter and the alteration of its carbon and nitrogen isotopic composition during microbial degradation. The decomposition rates determined with a double-exponential decay model show that the more reactive fraction of organic matter degrades at similar rates under oxic and anoxic conditions. However, under oxic conditions the proportion of organic matter resistent to degradation is much lower than under anoxic conditions, Within three months of incubation the delta(13)C of bulk organic matter decreased by 1.6parts per thousand with respect to the initial value. The depletion can be attributed to the selective preservation of C-13-depleted organic compounds. During anoxic decay, the delta(15)N values continuously decreased to about 3parts per thousand below the initial value. The decrease probably results from bacterial growth adding N-15-depleted biomass to the residual material. In the oxic experiment, delta(15)N values increased by more then 3parts per thousand, before decreasing to a value indistinguishable from the initial isotopic composition. The dissimilarity between oxic and anoxic conditions may be attributed to differences in the type. timing and degree of microbial activity and preferential degradation. In agreement with the anoxic incubation experiments, sediments from eutrophic Lake Lugano are. on average. depleted in C-13 (- 1.5parts per thousand) and N-15 (-1.2parts per thousand) with respect to sinking particulate organic matter collected during a long-term sediment trap study. Copyright (C) 2002 Elsevier Science Ltd.