Quantitative analysis of monoclonal antibodies by cation-exchange chromatofocusing

被引：33
|
作者：
Rozhkova, Anna [1 ]
机构：
[1] Solvias AG, Analyt Klybeck, CH-4002 Basel, Switzerland
来源：
JOURNAL OF CHROMATOGRAPHY A | 2009年 / 1216卷 / 32期
关键词：
Monoclonal antibody; Cation-exchange chromatofocusing; pH gradient; Acidic species quantitation; Isoform content determination; Lot release analysis; SIMPLE BUFFER MIXTURES; PERFORMANCE LIQUID-CHROMATOGRAPHY; PH GRADIENTS; MULTIDIMENSIONAL CHROMATOGRAPHY; SEPARATING PROTEINS; COLUMNS; HPLC; HETEROGENEITY; SUITABILITY; TRANSITIONS;
D O I：
10.1016/j.chroma.2009.06.045
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A robust cation-exchange chromatofocusing method was developed for the routine analysis of a recombinant humanized monoclonal IgG antibody. We compare the chromatofocusing method to the conventional cation-exchange chromatography (CEX) employing a salt gradient and show clear advantages of chromatofocusing over CEX We demonstrate the suitability of the present chromatofocusing method for its intended purpose by testing the validation characteristics. To our knowledge, this is the first chromatofocusing method developed for the routine analysis of monoclonal antibody charge species. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码：5989 / 5994
页数：6
相关论文
共 50 条
  • [21] Novel applications of chromatofocusing for the purification of monoclonal antibodies
    Liu, Yang
    Deldari, Sevda
    Guo, Hui
    Rao, Chittoor
    Bates, Ronald
    West, Jay
    Trejo, Kathleen
    Swanson, Ryan
    Ghose, Sanchayita
    Li, Zhengjian
    Frey, Douglas
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2019, 257
  • [22] CATION-EXCHANGE IN GANOPHYLLITE
    GUGGENHEIM, S
    EGGLETON, RA
    MINERALOGICAL MAGAZINE, 1986, 50 (357) : 517 - 520
  • [23] CATION-EXCHANGE RESINS
    MORTON, F
    LANCET, 1951, 260 (MAY12): : 1074 - 1075
  • [24] Rapid analysis of charge variants of monoclonal antibodies using non-linear salt gradient in cation-exchange high performance liquid chromatography
    Joshi, Varsha
    Kumar, Vijesh
    Rathore, Anurag S.
    JOURNAL OF CHROMATOGRAPHY A, 2015, 1406 : 175 - 185
  • [25] BIOT 339-Successful scale-up of pH elution on cation-exchange column for the purification of monoclonal antibodies
    Zaman, Ferhana
    Glynn, Judy
    Ramasubramanyan, Natrajan
    Mensah, Paul
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2007, 234
  • [26] Investigation of degradation processes in IgG1 monoclonal antibodies by limited proteolysis coupled with weak cation-exchange HPLC
    Lau, Hollis
    Pace, Danielle
    Yan, Boxu
    McGrath, Theresa
    Smallwood, Scott
    Patel, Ketaki
    Park, Jihea
    Park, SungAe S.
    Latypov, Ramil F.
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2010, 878 (11-12): : 868 - 876
  • [27] QUANTITATIVE CHROMATOGRAPHY OF POLYAMINES AND RELATED COMPOUNDS WITH CATION-EXCHANGE RESIN PAPER
    RINALDINI, LM
    ANALYTICAL BIOCHEMISTRY, 1970, 36 (02) : 352 - +
  • [28] QUANTITATIVE-ANALYSIS OF CATION-EXCHANGE RESINS BY INFRARED SPECTROPHOTOMETRY AND PYROLYSIS-GAS CHROMATOGRAPHY
    PARRISH, JR
    ANALYTICAL CHEMISTRY, 1975, 47 (12) : 1999 - 2003
  • [29] RAPID ANALYSIS OF CATION-EXCHANGE PROPERTIES IN ACIDIC SOILS
    QIU, XC
    ZHU, YQ
    SOIL SCIENCE, 1993, 155 (04) : 301 - 308
  • [30] QUANTITATIVE SEPARATION OF GALLIUM FROM OTHER ELEMENTS BY CATION-EXCHANGE CHROMATOGRAPHY
    VANDERWALT, TN
    STRELOW, FWE
    ANALYTICAL CHEMISTRY, 1983, 55 (02) : 212 - 216
12345