Embryonic stem cells from morula

被引:5
|
作者
Strelchenko, Nick [1 ]
Verlinsky, Yury [1 ]
机构
[1] Illinois Masonic Med Ctr, Inst Reprod Genet, Chicago, IL 60657 USA
来源
EMBRYONIC STEM CELLS | 2006年 / 418卷
关键词
D O I
10.1016/S0076-6879(06)18006-4
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
It has been shown that it is possible to establish human embryonic stem cell (hESC) lines from morula. Details of the aforementioned injection method of morula under blastocyst are described in this chapter. This chapter also discloses the application of simultaneous staining for two markers, TRA-2-39 and Oct-4, for characteristics of nondifferentiated hESC derived from morula and gives a method. Technical approaches of freezing morula-derived hESC are discussed.
引用
收藏
页码:93 / 108
页数:16
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