H1N1 Influenza A Virus Induced Apoptosis of Peripheral Blood Mononuclear Cell

被引：1

作者：

Zhang Heng ^{[1
]}

Wang Ge-Fei ^{[1
]}

Gao Yuan-Li ^{[1
]}

Chen Xiao-Xuan ^{[1
]}

Lin Gui-Mei ^{[1
]}

Li Kang-Sheng ^{[1
]}

机构：

[1] Shantou Univ, Coll Med, Dept Microbiol & Immunol, Key Immunopathol Lab Guangdong Prov, Shantou 515041, Peoples R China

来源：

PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS | 2009年 / 36卷 / 06期

基金：

中国国家自然科学基金;

关键词：

peripheral blood mononuclear cell; influenza A virus; apoptosis; p53; CHORION CELLS; INDUCTION; PROTEINS;

D O I：

10.3724/SP.J.1206.2008.00486

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Peripheral blood mononuclear cell (PBMC) was induced apoptosis when infected by influenza A virus. To observe the interaction between lymphocyte and macrophage, the lymphocyte and macrophage were induced by H1N1 subtype influenza vir-us. The result showed that apoptosis of PBMC including lymphocyte and macrophage was promoted by virus infection in the first 48 h, but the apoptosis of macrophage was inhibited after 48 h cultivation. According to fluorescent staining and flow cytometry there was no difference in apoptosis of lymphocyte after vir-us adsorption at different time. The result showed that there was no relationship between apoptosis and adsorption time. The apoptosis of lymphocyte and macrophage was detected when PFT-alpha was added, the result show that the apoptosis of lymphocyte and macrophage was inhibited. It hinted that p53 may be impact the apoptosis of lymphocyte and mononuclear macrophage that treated with influenza virus.

引用

页码：770 / 775

页数：6

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