Structural studies of an immunoenhancing water-soluble glucan isolated from hot water extract of an edible mushroom, Pleurotus florida, cultivar Assam Florida

被引:54
|
作者
Roy, Sadhan K. [1 ]
Das, Debsankar [1 ]
Mondal, Subhas [1 ]
Maiti, Debabrata [1 ]
Bhunia, Bibhas [2 ]
Maiti, Tapas K. [2 ]
Islam, Syed S. [1 ]
机构
[1] Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India
[2] Indian Inst Technol, Dept Biotechnol, Kharagpur 721302, W Bengal, India
关键词
Pleurotus florida; cv Assam Florida; Mushroom polysaccharide; Structure; NMR spectroscopy; Immunoactivation; SAJOR-CAJU; AQUEOUS EXTRACT; ANTITUMOR POLYSACCHARIDES; CHINESE MUSHROOM; (1->3)-BETA-D-GLUCAN; ELUCIDATION; BODIES;
D O I
10.1016/j.carres.2009.09.010
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An immunoenhancing polysaccharide isolated from the hot water extract of the fruiting bodies of an edible mushroom Pleurotus florida, cultivar Assam Florida, was found to consist of only D-glucose as a monosaccharide constituent. On the basis of total acid hydrolysis, methylation analysis, periodate oxidation, Smith degradation, and NMR experiments (H-1, C-13, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC), the structure of the repeating unit of the polysaccharide was established as [GRAPHICS] This glucan stimulates macrophages, splenocytes, and thymocytes. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:2596 / 2601
页数:6
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