Hyaluronic Acid and a Short Peptide Improve the Performance of a PCL Electrospun Fibrous Scaffold Designed for Bone Tissue Engineering Applications

被引:30
|
作者
Rachmiel, Dana [1 ,2 ]
Anconina, Inbar [2 ,3 ]
Rudnick-Glick, Safra [1 ,2 ]
Halperin-Sternfeld, Michal [1 ,2 ]
Adler-Abramovich, Lihi [1 ,2 ]
Sitt, Amit [2 ,3 ]
机构
[1] Tel Aviv Univ, Goldschleger Sch Dent Med, Sackler Fac Med, Dept Oral Biol, IL-6997801 Tel Aviv, Israel
[2] Tel Aviv Univ, Ctr Nanosci & Nanotechnol, IL-6997801 Tel Aviv, Israel
[3] Tel Aviv Univ, Dept Phys Chem, Sch Chem, Raymond & Beverly Sackler Fac Exact Sci, IL-6997801 Tel Aviv, Israel
基金
欧洲研究理事会; 以色列科学基金会;
关键词
bone tissue engineering; electrospinning; self-assembly; short peptide; hyaluronic acid; scaffolds;
D O I
10.3390/ijms22052425
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bone tissue engineering is a rapidly developing, minimally invasive technique for regenerating lost bone with the aid of biomaterial scaffolds that mimic the structure and function of the extracellular matrix (ECM). Recently, scaffolds made of electrospun fibers have aroused interest due to their similarity to the ECM, and high porosity. Hyaluronic acid (HA) is an abundant component of the ECM and an attractive material for use in regenerative medicine; however, its processability by electrospinning is poor, and it must be used in combination with another polymer. Here, we used electrospinning to fabricate a composite scaffold with a core/shell morphology composed of polycaprolactone (PCL) polymer and HA and incorporating a short self-assembling peptide. The peptide includes the arginine-glycine-aspartic acid (RGD) motif and supports cellular attachment based on molecular recognition. Electron microscopy imaging demonstrated that the fibrous network of the scaffold resembles the ECM structure. In vitro biocompatibility assays revealed that MC3T3-E1 preosteoblasts adhered well to the scaffold and proliferated, with significant osteogenic differentiation and calcium mineralization. Our work emphasizes the potential of this multi-component approach by which electrospinning, molecular self-assembly, and molecular recognition motifs are combined, to generate a leading candidate to serve as a scaffold for bone tissue engineering.
引用
收藏
页数:13
相关论文
共 50 条
  • [21] Electrospun fibrous silk fibroin/poly(L-lactic acid) scaffold for cartilage tissue engineering
    Liu, Weiwei
    Li, Zhengqiang
    Zheng, Lu
    Zhang, Xiaoyan
    Liu, Peng
    Yang, Ting
    Han, Bing
    TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 2016, 13 (05) : 516 - 526
  • [22] Electrospun fibrous silk fibroin/poly(L-lactic acid) scaffold for cartilage tissue engineering
    Weiwei Liu
    Zhengqiang Li
    Lu Zheng
    Xiaoyan Zhang
    Peng Liu
    Ting Yang
    Bing Han
    Tissue Engineering and Regenerative Medicine, 2016, 13 : 516 - 526
  • [23] Polycaprolactone fibrous electrospun scaffolds reinforced with copper doped wollastonite for bone tissue engineering applications
    Abudhahir, Mohamed
    Saleem, Azeena
    Paramita, Pragyan
    Kumar, Sukumar Dinesh
    Tze-Wen, Chung
    Selvamurugan, Nagarajan
    Moorthi, Ambigapathi
    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 2021, 109 (05) : 654 - 664
  • [24] An injectable click-crosslinked hyaluronic acid hydrogel modified with a BMP-2 mimetic peptide as a bone tissue engineering scaffold
    Park, Seung Hun
    Park, Joon Yeong
    Ji, Yun Bae
    Ju, Hyeon Jin
    Min, Byoung Hyun
    Kim, Moon Suk
    ACTA BIOMATERIALIA, 2020, 117 : 108 - 120
  • [25] Performance of 3D printed PCL/PLGA/HA biological bone tissue engineering scaffold
    Ma, Zhiyong
    Wang, Qifan
    Xie, Wenjia
    Ye, Wenjie
    Zhong, Linna
    Huge, Jile
    Wang, Ying
    POLYMER COMPOSITES, 2021, 42 (07) : 3593 - 3602
  • [26] Electrospun nano-fibrous bilayer scaffold prepared from polycaprolactone/gelatin and bioactive glass for bone tissue engineering
    Elkhouly, Hend
    Mamdouh, Wael
    El-Korashy, Dalia, I
    JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 2021, 32 (09)
  • [27] Electrospun nano-fibrous bilayer scaffold prepared from polycaprolactone/gelatin and bioactive glass for bone tissue engineering
    Hend Elkhouly
    Wael Mamdouh
    Dalia I. El-Korashy
    Journal of Materials Science: Materials in Medicine, 2021, 32
  • [28] Electrospun Biomimetic Fibrous Scaffold from Shape Memory Polymer of PDLLA-co-TMC for Bone Tissue Engineering
    Bao, Min
    Lou, Xiangxin
    Zhou, Qihui
    Dong, Wen
    Yuan, Huihua
    Zhang, Yanzhong
    ACS APPLIED MATERIALS & INTERFACES, 2014, 6 (04) : 2611 - 2621
  • [29] Graphene oxide-enriched poly(ε-caprolactone) electrospun nanocomposite scaffold for bone tissue engineering applications
    Mohammadi, Sepideh
    Shafiei, Seyedeh Sara
    Asadi-Eydivand, Mitra
    Ardeshir, Mahmoud
    Solati-Hashjin, Mehran
    JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS, 2017, 32 (03) : 325 - 342
  • [30] Electrospun poly(L-lactic acid)/hydroxyapatite composite fibrous scaffolds for bone tissue engineering
    Chuenjitkuntaworn, Boontharika
    Supaphol, Pitt
    Pavasant, Prasit
    Damrongsri, Damrong
    POLYMER INTERNATIONAL, 2010, 59 (02) : 227 - 235