Hyaluronic Acid and a Short Peptide Improve the Performance of a PCL Electrospun Fibrous Scaffold Designed for Bone Tissue Engineering Applications

被引:30
|
作者
Rachmiel, Dana [1 ,2 ]
Anconina, Inbar [2 ,3 ]
Rudnick-Glick, Safra [1 ,2 ]
Halperin-Sternfeld, Michal [1 ,2 ]
Adler-Abramovich, Lihi [1 ,2 ]
Sitt, Amit [2 ,3 ]
机构
[1] Tel Aviv Univ, Goldschleger Sch Dent Med, Sackler Fac Med, Dept Oral Biol, IL-6997801 Tel Aviv, Israel
[2] Tel Aviv Univ, Ctr Nanosci & Nanotechnol, IL-6997801 Tel Aviv, Israel
[3] Tel Aviv Univ, Dept Phys Chem, Sch Chem, Raymond & Beverly Sackler Fac Exact Sci, IL-6997801 Tel Aviv, Israel
基金
欧洲研究理事会; 以色列科学基金会;
关键词
bone tissue engineering; electrospinning; self-assembly; short peptide; hyaluronic acid; scaffolds;
D O I
10.3390/ijms22052425
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bone tissue engineering is a rapidly developing, minimally invasive technique for regenerating lost bone with the aid of biomaterial scaffolds that mimic the structure and function of the extracellular matrix (ECM). Recently, scaffolds made of electrospun fibers have aroused interest due to their similarity to the ECM, and high porosity. Hyaluronic acid (HA) is an abundant component of the ECM and an attractive material for use in regenerative medicine; however, its processability by electrospinning is poor, and it must be used in combination with another polymer. Here, we used electrospinning to fabricate a composite scaffold with a core/shell morphology composed of polycaprolactone (PCL) polymer and HA and incorporating a short self-assembling peptide. The peptide includes the arginine-glycine-aspartic acid (RGD) motif and supports cellular attachment based on molecular recognition. Electron microscopy imaging demonstrated that the fibrous network of the scaffold resembles the ECM structure. In vitro biocompatibility assays revealed that MC3T3-E1 preosteoblasts adhered well to the scaffold and proliferated, with significant osteogenic differentiation and calcium mineralization. Our work emphasizes the potential of this multi-component approach by which electrospinning, molecular self-assembly, and molecular recognition motifs are combined, to generate a leading candidate to serve as a scaffold for bone tissue engineering.
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页数:13
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