Fecal and Mucosal Microbiota Profiling in Irritable Bowel Syndrome and Inflammatory Bowel Disease

被引：139

作者：

Lo Presti, Alessandra ^{[1
]}

Zorzi, Francesca ^{[2
]}

Del Chierico, Federica ^{[3
]}

Altomare, Annamaria ^{[4
]}

Cocca, Silvia ^{[4
]}

Avola, Alessandra ^{[4
]}

De Biasio, Fabiola ^{[4
]}

Russo, Alessandra ^{[3
]}

Cella, Eleonora ^{[5
]}

Reddel, Sofia ^{[3
]}

Calabrese, Emma ^{[2
]}

Biancone, Livia ^{[2
]}

Monteleone, Giovanni ^{[2
]}

Cicala, Michele ^{[4
]}

Angeletti, Silvia ^{[6
]}

Ciccozzi, Massimo ^{[5
]}

Putignani, Lorenza ^{[3
,7
]}

Guarino, Michele Pier Luca ^{[4
]}

机构：

[1] Ist Super Sanita, Dept Infect Dis, Rome, Italy

[2] Univ Roma Tor Vergata, Dept Syst Med, Gastrointestinal Unit, Rome, Italy

[3] IRCCS, Human Microbiome Unit, Bambino Gesu Childrens Hosp, Rome, Italy

[4] Campus Biomed Univ, Unit Digest Dis, Rome, Italy

[5] Campus Biomed Univ, Unit Med Stat & Mol Epidemiol, Rome, Italy

[6] Campus Biomed Univ, Unit Clin Lab Sci, Rome, Italy

[7] IRCCS, Parasitol Unit, Bambino Gesu Childrens Hosp, Rome, Italy

来源：

FRONTIERS IN MICROBIOLOGY | 2019年 / 10卷

关键词：

gut microbiota; irritable bowel syndrome; inflammatory bowel disease; dysbiosis; inflammation; INVASIVE ESCHERICHIA-COLI; CROHNS-DISEASE; GUT MICROBIOTA; AKKERMANSIA-MUCINIPHILA; BILE-ACID; DYSBIOSIS; PREVALENCE; PATHOGENESIS; BACTERIA; IMPACT;

D O I：

10.3389/fmicb.2019.01655

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An imbalance in the bacterial species resulting in the loss of intestinal homeostasis has been described in inflammatory bowel diseases (IBD) and irritable bowel syndrome (IBS). In this prospective study, we investigated whether IBD and IBS patients exhibit specific changes in richness and distribution of fecal and mucosal-associated microbiota. Additionally, we assessed potential 16S rRNA gene amplicons biomarkers for IBD, IBS, and controls (CTRLs) by comparison of taxonomic composition. The relative abundance of bacteria, at phylum and genus/species levels, and the bacterial diversity were determined through 16S rRNA sequence-based fecal and mucosal microbiota analysis. Linear discriminant analysis effect size (LEfSe) was used for biomarker discovery associated to IBD and IBS as compared to CTRLs. In fecal and mucosal samples, the microbiota richness was characterized by a microbial diversity reduction, going from CTRLs to IBS to IBD. beta-diversity analysis showed a clear separation between IBD and CTRLs and between IBD and IBS with no significant separation between IBS and CTRLs. beta-diversity showed a clear separation between mucosa and stool samples in all the groups. In IBD, there was no difference between inflamed and not inflamed mucosa. Based upon the LEfSe data, the Anaerostipes and Ruminococcaceae were identified as the most differentially abundant bacterial taxa in CTRLs. Erysipelotrichi was identified as potential biomarker for IBS, while Gammaproteobacteria, Enterococcus, and Enterococcaceae for IBD. This study provides an overview of the alterations of microbiota and may aid in identifying potential 16S rRNA gene amplicons mucosal biomarkers for IBD and IBS.

引用

页数：14

共 50 条

[21] Probiotics, irritable bowel syndrome, and inflammatory bowel disease
Martin H. Floch
[J]. Current Treatment Options in Gastroenterology, 2003, 6 (4) : 283 - 288
[22] Which is it: Irritable bowel syndrome or inflammatory bowel disease?
Heitkemper, M
[J]. JOURNAL OF WOMENS HEALTH & GENDER-BASED MEDICINE, 2001, 10 (04): : 394 - 394
[23] INFLAMMATORY BOWEL-DISEASE OR IRRITABLE BOWEL SYNDROME
THOMPSON, WG
[J]. CANADIAN MEDICAL ASSOCIATION JOURNAL, 1982, 127 (04) : 271 - 272
[24] Irritable bowel syndrome, inflammatory bowel disease and the microbiome
Major, Giles
Spiller, Robin
[J]. CURRENT OPINION IN ENDOCRINOLOGY DIABETES AND OBESITY, 2014, 21 (01) : 15 - 21
[25] Overlapping irritable bowel syndrome and inflammatory bowel disease
Gajula, Prianka
Quigley, Eamonn M.
[J]. MINERVA GASTROENTEROLOGICA E DIETOLOGICA, 2019, 65 (02) : 107 - 115
[26] Irritable Bowel Syndrome and Inflammatory Bowel Disease: Is There an Overlap?
Quigley, Eamonn M. M.
Shanahan, Fergus
[J]. PRACTICAL GASTROENTEROLOGY, 2010, 34 (11) : 31 - 37
[27] Fecal Microbiota Transfer May Increase Irritable Bowel Syndrome and Inflammatory Bowel Diseases-Associated Bacteria
Konstantinov, Sergey R.
Peppelenbosch, Maikel P.
[J]. GASTROENTEROLOGY, 2013, 144 (04) : E19 - E20
[28] Fecal microbiota transplantation as a new therapy: from Clostridioides difficile infection to inflammatory bowel disease, irritable bowel syndrome, and colon cancer
Borody, Thomas J.
Eslick, Guy D.
Clancy, Robert L.
[J]. CURRENT OPINION IN PHARMACOLOGY, 2019, 49 : 43 - 51
[29] Specificities of the fecal microbiota in inflammatory bowel disease
Sokol, H
Seksik, P
Rigottier-Gois, L
Lay, C
Lepage, P
Podglajen, I
Marteau, P
Doré, J
[J]. INFLAMMATORY BOWEL DISEASES, 2006, 12 (02) : 106 - 111
[30] Fecal Microbiota Transplantation in Inflammatory Bowel Disease
Reinisch, Walter
[J]. DIGESTIVE DISEASES, 2017, 35 (1-2) : 123 - 126

← 1 2 3 4 5 →