Effect of nanovesicle-encapsulated nisin on growth of Listeria monocytogenes in milk

被引：88

作者：

Malheiros, Patricia da Silva ^{[1
]}

Daroit, Daniel Joner ^{[1
]}

da Silveira, Nadya Pesce ^{[2
]}

Brandelli, Adriano ^{[1
]}

机构：

[1] Univ Fed Rio Grande do Sul, ICTA, Dept Ciencia Alimentos, Lab Bioquim & Microbiol Aplicada, BR-91501970 Porto Alegre, RS, Brazil

[2] Univ Fed Rio Grande do Sul, Inst Quim, Lab Instrumentacao & Dinam Mol, BR-91501970 Porto Alegre, RS, Brazil

来源：

FOOD MICROBIOLOGY | 2010年 / 27卷 / 01期

关键词：

Liposome; Encapsulation; Nisin; Listeria monocytogenes; Milk; LIPOSOMES; PRESERVATION; ACID; INHIBITION; STABILITY; LYSOZYME; EFFICACY; CHEESE; SIZE; FAT;

D O I：

10.1016/j.fm.2009.09.013

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

commercial nisin was encapsulated in nanovesicles (mean diameter 140 nm) prepared from partially purified soy lecithin. Nisin-loaded liposomes and unencapsulated (free) nisin were initially tested in BHI medium and skim milk inoculated with Listeria monocytogenes and incubated for 48 h at 30 degrees C. At such abuse temperature conditions, free nisin showed better inhibitory than the liposomal counterparts. Subsequently, the effect of encapsulated or free nisin was evaluated in combination with refrigeration (7 +/- 1 degrees C) in both whole (3.25% fat) and skim (0% fat) milk for up to 14 day. A decrease of 3-4 log cycles in L. monocytogenes counts was observed for free and encapsulated nisin at 0.5 mg/ml concentration. Liposome encapsulation of antimicrobial peptides may be important to overcome stability issues and interaction with food components. The utilization of nanovesicle-encapsulated nisin in combination with low temperatures appeared to be effective to control L monocytogenes in milk, emphasizing the importance of hurdle technology to assure food safety. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：175 / 178

页数：4

共 50 条

[21] Dynamic modeling of Listeria monocytogenes growth in pasteurized milk
Xanthiakos, K.
Simos, D.
Angelidis, A. S.
Nychas, G. J. -E.
Koutsoumanis, K.
JOURNAL OF APPLIED MICROBIOLOGY, 2006, 100 (06) : 1289 - 1298
[22] Dynamic modeling of Listeria monocytogenes growth in pasteurized milk
Xanthiakos, K.
Simos, D.
Angelidis, A.S.
Nychas, G.J.-E.
Koutsoumanis, K.
Journal of Applied Microbiology, 2006, 100 (06): : 1289 - 1298
[23] Sensitivity of six strains of Listeria monocytogenes to nisin
Ukuku, DO
Shelef, LA
JOURNAL OF FOOD PROTECTION, 1997, 60 (07) : 867 - 869
[24] Occurrence and growth of Listeria monocytogenes in packaged raw milk
Castro, Hanna
Ruusunen, Marjo
Lindstrom, Miia
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2017, 261 : 1 - 10
[25] COMPARISON OF Listeria monocytogenes GROWTH IN WHOLE AND SKIM MILK
Garcia, M. J.
Pascual, L.
Barberis, L.
BIOCELL, 2013, 37 (03) : A122 - A122
[26] In vivo activity of Nisin A and Nisin V against Listeria monocytogenes in mice
Campion, Alicia
Casey, Pat G.
Field, Des
Cotter, Paul D.
Hill, Colin
Ross, R. Paul
BMC MICROBIOLOGY, 2013, 13
[27] RESISTANCE OF LISTERIA-MONOCYTOGENES TO THE BACTERIOCIN NISIN
DAVIES, EA
ADAMS, MR
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1994, 21 (04) : 341 - 347
[28] Mechanism of Synergistic Inhibition of Listeria monocytogenes Growth by Lactic Acid, Monolaurin, and Nisin
Tokarskyy, Oleksandr
Marshall, Douglas L.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (23) : 7126 - 7129
[29] Lactobionic acid enhances the synergistic effect of nisin and thymol against Listeria monocytogenes Scott A in tryptic soy broth and milk
Chen, Huaiqiong
Zhong, Qixin
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2017, 260 : 36 - 41
[30] Effects of Pseudomonas fluorescens on the growth of Listeria monocytogenes and Listeria innocua in skimmed milk
Quinto, EJ
Franco, CM
Fente, CA
Vazquez, BI
Cepeda, A
ARCHIV FUR LEBENSMITTELHYGIENE, 1996, 47 (05): : 107 - 110

← 1 2 3 4 5 →