Monitoring membrane viscosity in differentiating stem cells using BODIPY-based molecular rotors and FLIM

被引：42
|
作者：
Kashirina, Alena S. [1 ]
Lopez-Duarte, Ismael [2 ]
Kubankova, Marketa [2 ]
Gulin, Alexander A. [3 ,4 ]
Dudenkova, Varvara V. [1 ]
Rodimova, Svetlana A. [1 ,5 ]
Torgomyan, Hayk G. [1 ]
Zagaynova, Elena V. [1 ,5 ]
Meleshina, Aleksandra V. [1 ]
Kuimova, Marina K. [2 ]
机构：
[1] Privolzhsky Res Med Univ, 10-1 Minin & Pozharsky Sq, Nizhnii Novgorod 603950, Russia
[2] Imperial Coll London, Dept Chem, Mol Sci Res Hub, White City Campus, London W12 0BZ, England
[3] Russian Acad Sci FRCCP RAS, NN Semenov Fed Res Ctr Chem Phys, Kosygin St 4, Moscow 119991, Russia
[4] Lomonosov Moscow State Univ, Dept Chem, Leninskiye Gory 1-3, Moscow 119991, Russia
[5] Lobachevsky State Univ Nizhny Novgorod, 23 Gagarin Ave, Novgorod 603950, Nizhny Novgorod, Russia
来源：
SCIENTIFIC REPORTS | 2020年 / 10卷 / 01期
基金：
俄罗斯基础研究基金会; 英国工程与自然科学研究理事会; 俄罗斯科学基金会;
关键词：
PLASMA-MEMBRANES; PHASES; MICROSCOPY; SEPARATION;
D O I：
10.1038/s41598-020-70972-5
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Membrane fluidity plays an important role in many cell functions such as cell adhesion, and migration. In stem cell lines membrane fluidity may play a role in differentiation. Here we report the use of viscosity-sensitive fluorophores based on a BODIPY core, termed "molecular rotors", in combination with Fluorescence Lifetime Imaging Microscopy, for monitoring of plasma membrane viscosity changes in mesenchymal stem cells (MSCs) during osteogenic and chondrogenic differentiation. In order to correlate the viscosity values with membrane lipid composition, the detailed analysis of the corresponding membrane lipid composition of differentiated cells was performed by time-of-flight secondary ion mass spectrometry. Our results directly demonstrate for the first time that differentiation of MSCs results in distinct membrane viscosities, that reflect the change in lipidome of the cells following differentiation.
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页数：12
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