Characterization of Plum pox virus PPV-BT-H isolated from naturally infected blackthorn (Prunus spinosa L.) in Hungary

被引：8

作者：

Salamon, P ^{[1
]}

Palkovics, L ^{[1
]}

机构：

[1] Agr Biotechnol Ctr, Environm Biosafety Inst, H-2101 Godollo, Hungary

来源：

EUROPEAN JOURNAL OF PLANT PATHOLOGY | 2002年 / 108卷 / 09期

关键词：

coat protein sequences; potyvirus; PPV; virus reservoir;

D O I：

10.1023/A:1021265023657

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Plum pox virus (PPV) was found naturally infecting blackthorn (Prunus spinosa L.) plants in different regions in Hungary. The virus was identified on the basis of reactions with indicator plants, by DAS-ELISA tests and by RT-PCR. PPV isolated from blackthorn (PPV-BT-H) caused yellow lesions with a necrotic centre on Chenopodium foetidum L. indicating that it belongs to the intermediate pathotype. The coat protein gene of the blackthorn isolate was cloned, sequenced and compared with other PPV sequences. The BT-H isolate and the Hungarian plum isolate SK68 belong to different subclusters of the M group in contrast to the Hungarian almond isolate (PA) which belongs to the D group. Detecting PPV on blackthorn demonstrated that this plant may be an important source and reservoir for PPV in Hungary.

引用

页码：903 / 907

页数：5

共 15 条

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