Catalytic Amplification of Electrochemical Signal in Homogeneous Solution Using an Entropy-driven DNA Circuit

被引：1

作者：

Kitamura, Yusuke ^{[1
]}

Yoshimura, Keisuke ^{[1
]}

Kuramoto, Ryo ^{[1
]}

Katsuda, Yousuke ^{[1
]}

Ihara, Toshihiro ^{[1
]}

机构：

[1] Kumamoto Univ, Fac Adv Sci & Technol, Div Mat Sci, Chuo Ku, 2-39-1 Kurokami, Kumamoto 8608555, Japan

来源：

ANALYTICAL SCIENCES | 2021年 / 37卷 / 03期

关键词：

Nucleic acids; electrochemical biosensor; inclusion complex; ferrocene; cyclodextrin; homogeneous assay; DNA circuit; signal amplification; COMPLEX; HYBRIDIZATION; CYCLODEXTRIN; WALKER;

D O I：

10.2116/analsci.20SCN04

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The electrochemical signal from ferrocene on a DNA probe was successfully modulated in a homogeneous solution by the template-directed formation and dissociation of an inclusion complex with beta-cyclodextrin on another probe. The electrochemical response was amplified by combining with a DNA circuit, in which the target DNA served as a catalyst. This system did not require any modification of a complementary DNA with the ferrocene-modified probe on the electrode surface to separate the bound/free probe for the detection of 200 nM target DNA.

引用

页码：533 / 537

页数：5

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