Fluorescence in situ hybridization (FISH) for direct visualization of microorganisms

被引：542
|
作者：
Moter, A [1 ]
Göbel, UB [1 ]
机构：
[1] Humboldt Klinikum Univ, Charite, Inst Mikrobiol & Hyg, D-10117 Berlin, Germany
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2000年 / 41卷 / 02期
关键词：
FISH; fluorescence; in situ hybridization; 16S rRNA; probes;
D O I：
10.1016/S0167-7012(00)00152-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
As a technique allowing simultaneous visualization, identification, enumeration and localization of individual microbial cells, fluorescence in situ hybridization (FISH) is useful for many applications in all fields of microbiology. FISH not only allows the detection of culturable microorganisms, but also of yet-to-be cultured (so-called unculturable) organisms, and can therefore help in understanding complex microbial communities. In this review, methodological aspects, as well as problems and pitfalls of FISH are discussed in an examination of past, present and future applications. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：85 / 112
页数：28
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