A functional quantitative polymerase chain reaction assay for ricin, Shiga toxin, and related ribosome-inactivating proteins

被引：43

作者：

Melchior, William B., Jr. ^{[1
]}

Tolleson, William H. ^{[1
]}

机构：

[1] US FDA, Div Biochem Toxicol, Natl Ctr Toxicol Res, Jefferson, AR 72079 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2010年 / 396卷 / 02期

关键词：

Ricin; Ribosome-inactivating proteins; Quantitative PCR; Reverse transcriptase; LOCKED NUCLEIC-ACID; A-CHAIN; RNA; LOOP; DNA; ELECTROCHEMILUMINESCENCE; INHIBITORS; PRIMERS;

D O I：

10.1016/j.ab.2009.09.024

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The potent toxins ricin, abrin, and other ribosome-inactivating proteins deadenylate a specific base in 28S ribosomal RNA that destroys ribosomes and leads to cell death. We have taken advantage of the fact that reverse transcriptase preferentially inserts an adenine opposite to an abasic site in RNA to create a quantitative polymerase chain reaction (PCR) assay to detect the damage. This assay detects as little as 30 pg of ricin. We used the assay to study enzymatic properties of ricin such as pH and temperature optima (pH 4.5-5.0 and 60 degrees C). Published by Elsevier Inc.

引用

页码：204 / 211

页数：8

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