Single-cell chromatin accessibility landscape identifies tissue repair program in human regulatory T cells

被引：89

作者：

Delacher, Michael ^{[1
,2
,3
,4
]}

Simon, Malte ^{[5
,6
]}

Sanderink, Lieke ^{[1
,2
]}

Hotz-Wagenblatt, Agnes ^{[7
]}

Wuttke, Marina ^{[1
,2
]}

Schambeck, Kathrin ^{[1
,2
]}

Schmidleithner, Lisa ^{[1
,2
]}

Bittner, Sebastian ^{[1
,2
]}

Pant, Asmita ^{[1
,2
]}

Ritter, Uwe ^{[1
,2
]}

Hehlgans, Thomas ^{[1
,2
]}

Riegel, Dania ^{[1
]}

Schneider, Verena ^{[8
,9
]}

Groeber-Becker, Florian Kai ^{[8
,9
]}

Eigenberger, Andreas ^{[10
]}

Gebhard, Claudia ^{[1
]}

Strieder, Nicholas ^{[1
]}

Fischer, Alexander ^{[1
,11
]}

Rehli, Michael ^{[1
,11
]}

Hoffmann, Petra ^{[1
,11
]}

Edinger, Matthias ^{[1
,11
]}

Strowig, Till ^{[12
,14
,15
]}

Huehn, Jochen ^{[13
,15
]}

Schmidl, Christian ^{[1
]}

Werner, Jens M. ^{[16
]}

Prantl, Lukas ^{[10
]}

Brors, Benedikt ^{[6
,17
,18
]}

Imbusch, Charles D. ^{[6
]}

Feuerer, Markus ^{[1
,2
]}

机构：

[1] Regensburg Ctr Intervent Immunol RCI, Regensburg, Germany

[2] Univ Regensburg, Chair Immunol, D-93053 Regensburg, Germany

[3] Univ Med Ctr Mainz, Inst Immunol, D-55131 Mainz, Germany

[4] Univ Med Ctr Mainz, Res Ctr Immunotherapy, D-55131 Mainz, Germany

[5] Heidelberg Univ, Fac Biosci, D-69120 Heidelberg, Germany

[6] German Canc Res Ctr, Div Appl Bioinformat, D-69120 Heidelberg, Germany

[7] German Canc Res Ctr, Core Facil Omics IT & Data Management ODCF, D-69120 Heidelberg, Germany

[8] Univ Hosp Wurzburg, Dept Tissue Engn & Regenerat Med TERM, D-97070 Wurzburg, Germany

[9] Translat Ctr Regenerat Therapies TLZ RT, Fraunhofer Inst Silicate Res ISC, D-97082 Wurzburg, Germany

[10] Univ Hosp Regensburg, Dept Plast Hand & Reconstruct Surg, D-93053 Regensburg, Germany

[11] Univ Hosp Regensburg, Dept Internal Med 3, D-93053 Regensburg, Germany

[12] Helmholtz Ctr Infect Res, Dept Microbial Immune Regulat, D-38124 Braunschweig, Germany

[13] Helmholtz Ctr Infect Res, Dept Expt Immunol, D-38124 Braunschweig, Germany

[14] Hannover Med Sch, D-30625 Hannover, Germany

[15] Hannover Med Sch, Cluster Excellence 2155, RESIST, D-30625 Hannover, Germany

[16] Univ Hosp Regensburg, Dept Surg, D-93053 Regensburg, Germany

[17] Natl Ctr Tumor Dis NCT, D-69120 Heidelberg, Germany

[18] German Canc Res Ctr, German Canc Consortium DKTK, D-69120 Heidelberg, Germany

来源：

IMMUNITY | 2021年 / 54卷 / 04期

基金：

欧洲研究理事会;

关键词：

GENOME BROWSER; POPULATION; ALIGNMENT; LINEAGE; BATF;

D O I：

10.1016/j.immuni.2021.03.007

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Murine regulatory T (Treg) cells in tissues promote tissue homeostasis and regeneration. We sought to identify features that characterize human Treg cells with these functions in healthy tissues. Single-cell chromatin accessibility profiles of murine and human tissue Treg cells defined a conserved, microbiota-independent tissue-repair Treg signature with a prevailing footprint of the transcription factor BATF. This signature, combined with gene expression profiling and TCR fate mapping, identified a population of tissue-like Treg cells in human peripheral blood that expressed BATF, chemokine receptor CCR8 and HLA-DR. Human BATF(+) CCR8(+) Treg cells from normal skin and adipose tissue shared features with nonlymphoid T follicular helper-like (Tfh-like) cells, and induction of a Tfh-like differentiation program in naive human Treg cells partially recapitulated tissue Treg regenerative characteristics, including wound healing potential. Human BATF(+) CCR8(+) Treg cells from healthy tissue share features with tumor-resident Treg cells, highlighting the importance of understanding the context-specific functions of these cells.

引用

页码：702 / +

页数：36

共 50 条

[21] Profiling single-cell chromatin accessibility in plants
Marand, Alexandre P.
Zhang, Xuan
Nelson, Julie
dos Reis, Pedro Augusto Braga
Schmitz, Robert J.
STAR PROTOCOLS, 2021, 2 (03):
[22] Spatially mapped single-cell chromatin accessibility
Thornton, Casey A.
Mulqueen, Ryan M.
Torkenczy, Kristof A.
Nishida, Andrew
Lowenstein, Eve G.
Fields, Andrew J.
Steemers, Frank J.
Zhang, Wenri
McConnell, Heather L.
Woltjer, Randy L.
Mishra, Anusha
Wright, Kevin M.
Adey, Andrew C.
NATURE COMMUNICATIONS, 2021, 12 (01)
[23] The Chromatin Accessibility Landscape of Peripheral Blood Mononuclear Cells in Patients With Systemic Lupus Erythematosus at Single-Cell Resolution
Yu, Haiyan
Hong, Xiaoping
Wu, Hongwei
Zheng, Fengping
Zeng, Zhipeng
Dai, Weier
Yin, Lianghong
Liu, Dongzhou
Tang, Donge
Dai, Yong
FRONTIERS IN IMMUNOLOGY, 2021, 12
[24] IReNA: Integrated regulatory network analysis of single-cell transcriptomes and chromatin accessibility profiles
Jiang, Junyao
Lyu, Pin
Li, Jinlian
Huang, Sunan
Tao, Jiawang
Blackshaw, Seth
Qian, Jiang
Wang, Jie
ISCIENCE, 2022, 25 (11)
[25] Single-Cell Transcriptomics of Regulatory T Cells Reveals Trajectories of Tissue Adaptation
Miragaia, Ricardo J.
Gomes, Tomas
Chomka, Agnieszka
Jardine, Laura
Riedel, Angela
Hegazy, Ahmed N.
Whibley, Natasha
Tucci, Andrea
Chen, Xi
Lindeman, Ida
Emerton, Guy
Krausgruber, Thomas
Shields, Jacqueline
Haniffa, Muzlifah
Powrie, Fiona
Teichmann, Sarah A.
IMMUNITY, 2019, 50 (02) : 493 - +
[26] Single-cell ATAC-seq maps the comprehensive and dynamic chromatin accessibility landscape of CAR-T cell dysfunction
Penglei Jiang
Zhaoru Zhang
Yongxian Hu
Zuyu Liang
Yingli Han
Xia Li
Xin Zeng
Hao Zhang
Meng Zhu
Jian Dong
He Huang
Pengxu Qian
Leukemia, 2022, 36 : 2656 - 2668
[27] Single-cell ATAC-seq maps the comprehensive and dynamic chromatin accessibility landscape of CAR-T cell dysfunction
Jiang, Penglei
Zhang, Zhaoru
Hu, Yongxian
Liang, Zuyu
Han, Yingli
Li, Xia
Zeng, Xin
Zhang, Hao
Zhu, Meng
Dong, Jian
Huang, He
Qian, Pengxu
LEUKEMIA, 2022, 36 (11) : 2656 - 2668
[28] Single-cell RNA-seq and chromatin accessibility profiling decipher the heterogeneity of mouse γδ T cells
Li, Zhenhua
Yang, Quanli
Tang, Xin
Chen, Yiming
Wang, Shanshan
Qi, Xiaojie
Zhang, Yawen
Liu, Zonghua
Luo, Jing
Liu, Hui
Ba, Yongbing
Guo, Lianxia
Wu, Baojian
Huang, Fang
Cao, Guangchao
Yin, Zhinan
SCIENCE BULLETIN, 2022, 67 (04) : 408 - 426
[29] SINGLE-CELL CHROMATIN ACCESSIBILITY ANALYSIS IDENTIFIES EPIGENETIC EVOLUTION IN RELAPSED ACUTE MYELOID LEUKEMIA
Nuno, Kevin
Azizi, Armon
Kohnke, Thomas
Majeti, Ravi
EXPERIMENTAL HEMATOLOGY, 2021, 100 : S56 - S56
[30] Single-cell RNA sequencing identifies inflammatory tissue T cells in eosinophilic esophagitis
Wen, Ting
Aronow, Bruce J.
Rochman, Yrina
Rochman, Mark
Kiran, K. C.
Dexheimer, Phil J.
Putnam, Philip
Mukkada, Vincent
Foote, Heather
Rehn, Kira
Darko, Sam
Douek, Daniel
Rothenberg, Marc E.
JOURNAL OF CLINICAL INVESTIGATION, 2019, 129 (05): : 2014 - 2028

← 1 2 3 4 5 →