Identification of polymorphisms and association analysis with meat quality traits in the porcine KIAA1717 and HUMMLC2B genes

被引:7
|
作者
Xu, D. Q. [1 ]
Liu, M. [1 ]
Xiong, Y. Z. [1 ]
Deng, C. Y. [1 ]
Jiang, S. W. [1 ]
Li, J. L. [1 ]
Zuo, B. [1 ]
Lei, M. G. [1 ]
Li, F. E. [1 ]
Zheng, R. [1 ]
机构
[1] Huazhong Agr Univ, Coll Anim Sci, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan 430070, Peoples R China
基金
中国国家自然科学基金;
关键词
H3-K4-specific methyltransferase; myosin regulatory light chain 2; pigs; polymorphism; meat quality traits;
D O I
10.1016/j.livsci.2006.07.005
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Skeletal muscle genes are potential candidates for production and meat quality. Screening a subtracted cDNA library constructed with mRNA obtained from longissimus dorsi muscles of F1 hybrids Landrace x Yorkshire and their female parents Yorkshire, we isolated two partial sequences coding for the H3-K4-specific methyltransferase (KIAA1717) and skeletal muscle myosin regulatory light chain (HUMMLC2B) genes. Database search revealed KIAA1717 and HUMMLC2B encoded proteins with SET domain and EF-hand calcium binding motif, respectively. In the present work we identified their partial polymorphisms and two SNPs, one (C1354T) at the 3' untranslated region (UTR) of KIAA1717 and one (A345G) at the SINE (PRE-1) element of HUMMLC2B, both created/disrupted a restriction site for endonuclease Msp I. The selected pigs were genotyped at the KIAA1717 C1354T and HUMMLC2B A345G sites by means of a PCR-RFLP protocol. Significant associations were observed for the KIAA1717 C1354T polymorphic site with meat marbling (longissimus dot-is (p < 0.05), biceps femoris (p < 0.01)) and intramuscular fat (p < 0.01). HUMMLC2B A345G were significantly associated with meat pH (longissimus doris (p < 0.05), biceps femoris (p < 0.01)), drip loss (p < 0.01), water holding capacity (p < 0.01) and meat color value (longissimus doris (P < 0.01), biceps femoris (p < 0.05)). Further studies are needed to confirm these preliminary results. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:96 / 101
页数:6
相关论文
共 50 条
  • [31] Investigation of theCEBPAgene expression pattern and association analysis of its polymorphisms with meat quality traits in chickens
    Cao, Haiyue
    Wen, Yaya
    Xu, XiuLi
    Liu, Ke
    Liu, Honghua
    Tan, Yuge
    Zhou, Wei
    Mao, Haiguang
    Dong, Xinyang
    Xu, Ningying
    Yin, Zhaozheng
    ANIMAL BIOTECHNOLOGY, 2022, 33 (03) : 448 - 456
  • [33] Association study of molecular polymorphisms in candidate genes related to stress responses with production and meat quality traits in pigs
    Terenina, E.
    Babigumira, B. M.
    Le Mignon, G.
    Bazovkina, D.
    Rousseau, S.
    Salin, F.
    Bendixen, C.
    Mormede, P.
    DOMESTIC ANIMAL ENDOCRINOLOGY, 2013, 44 (02) : 81 - 97
  • [34] Identification of candidate genes associated with porcine meat color traits by genome-wide transcriptome analysis
    Li, Bojiang
    Dong, Chao
    Li, Pinghua
    Ren, Zhuqing
    Wang, Han
    Yu, Fengxiang
    Ning, Caibo
    Liu, Kaiqing
    Wei, Wei
    Huang, Ruihua
    Chen, Jie
    Wu, Wangjun
    Liu, Honglin
    SCIENTIFIC REPORTS, 2016, 6
  • [35] Identification of Novel Polymorphisms in Porcine Ring Finger Protein 4 and Matrix Metalloproteinase 9 Genes and Association Analysis with Litter Size Traits
    Niu Bu-yue
    Lan Xiao-ming
    Xiong Yuan-zhu
    Wang Xi-biao
    Journal of Northeast Agricultural University(English Edition), 2016, 23 (03) : 31 - 38
  • [36] Identification of candidate genes associated with porcine meat color traits by genome-wide transcriptome analysis
    Bojiang Li
    Chao Dong
    Pinghua Li
    Zhuqing Ren
    Han Wang
    Fengxiang Yu
    Caibo Ning
    Kaiqing Liu
    Wei Wei
    Ruihua Huang
    Jie Chen
    Wangjun Wu
    Honglin Liu
    Scientific Reports, 6
  • [37] Association Analysis of Polymorphism in Intron-10 of Porcine HK2 Gene with Meat Quality and Carcass Traits
    Wang Jun
    Deng Chang-Yan
    Xiong Yuan-Zhu
    Zuo Bo
    JOURNAL OF ANIMAL AND VETERINARY ADVANCES, 2012, 11 (08): : 1158 - 1161
  • [38] Association of CRTC2 gene polymorphisms with growth and meat quality traits of Qinchuan cattle
    Xu, H. C.
    Gui, L. S.
    Song, N.
    Zhang, Y. Y.
    Wang, H. C.
    Zan, L. S.
    GENETICS AND MOLECULAR RESEARCH, 2015, 14 (04) : 12912 - 12920
  • [39] Polymorphisms in PEG10 and PPP1R9A genes are associated with porcine carcass and meat quality traits
    Qiao, Mu
    Wu, Huayu
    Zhang, Fengwei
    Liu, Guisheng
    Wu, Junjing
    Peng, Xianwen
    Mei, Shuqi
    ANIMAL GENETICS, 2016, 47 (02) : 270 - 270
  • [40] Association and expression analysis of porcine HNF1A gene related to meat and carcass quality traits
    Kayan, Autchara
    Uddin, Muhammad Jasim
    Kocamis, Hakan
    Tesfaye, Dawit
    Looft, Christian
    Tholen, Ernst
    Schellander, Karl
    Cinar, Mehmet Ulas
    MEAT SCIENCE, 2013, 94 (04) : 474 - 479