Integrated Clarification and Purification of Monoclonal Antibodies by Membrane Based Separation of Aqueous Two-Phase Systems

被引:14
|
作者
Kruse, Thomas [1 ,2 ]
Schmidt, Axel [1 ]
Kampmann, Markus [2 ]
Strube, Jochen [1 ]
机构
[1] Tech Univ Clausthal, Inst Separat & Proc Technol, Leibnizstr 15, D-38678 Clausthal Zellerfeld, Germany
[2] Sartorius Sted Biotech GmbH, August Spindler Str 11, D-37079 Gottingen, Germany
关键词
aqueous two-phase extraction; phase separation; downstream; clarification; membrane; CENTRIFUGAL PARTITIONING CHROMATOGRAPHY; EXTRACTION; TECHNOLOGY; OPTIMIZATION; PERFORMANCE; PLATFORM; DESIGN;
D O I
10.3390/antib8030040
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Therapeutic monoclonal antibodies (mAb) are used for the treatment of numerous serious diseases, which have led to an increasing demand over the last decades. Increased cell density and mAb titer of the cultivation broth lead to great challenges for the subsequent clarification and capture operations in the downstream process. As an alternative approach to the conventional downstream process, a selective mAb extraction via an aqueous two-phase system (ATPS) directly from the cultivation broth of a mAb producing industrial relevant chinese hamster ovary (CHO) cell line was investigated. An efficient purification of the mAb was accomplished by the ATPS composition. The phase separation was realized by a newly developed membrane based phase separator. Moreover, a complete cell removal was integrated into this process by the used membrane. A selectivity between both phases was achieved by membrane modification. Yields up to 93% in the light phase and removal of process related impurities were obtained after aqueous two-phase extraction (ATPE). Phase separation performance as well as contact angles on the membrane were characterized for different ATPS. ATPE directly from the cultivation broth in combination with the new membrane based phase separation led to a mAb yield of 78% with a simultaneous reduction of deoxyribonucleic acid (DNA) and host cell protein (HCP) load.
引用
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页数:17
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