METHODS OF SITE-SPECIFIC LABELING OF RNA WITH FLUORESCENT DYES

被引：43
|
作者：
Solomatin, Sergey [1 ]
Herschlag, Daniel [1 ,2 ]
机构：
[1] Stanford Univ, Dept Biochem, Stanford, CA 94305 USA
[2] Stanford Univ, Dept Chem, Stanford, CA 94305 USA
来源：
METHODS IN ENZYMOLOGY, VOL 469: BIOPHYSICAL, CHEMICAL, AND FUNCTIONAL PROBES OF RNA STRUCTURE, INTERACTIONS AND FOLDING, PT B | 2009年 / 469卷
关键词：
SINGLE-MOLECULE; TERTIARY INTERACTIONS; DNA; DYNAMICS; KINETICS; DOCKING; THERMODYNAMICS; COOPERATIVITY; HETEROGENEITY; TETRALOOP;
D O I：
10.1016/S0076-6879(09)69003-0
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Single molecule fluorescence techniques offer unique insights into mechanisms of conformational changes of RNA. Knowing how to make fluorescently labeled RNA molecules and understanding potential limitations of different labeling strategies is essential for successful implementation of single molecule fluorescence techniques. This chapter offers a step by step overview of the process of obtaining RNA constructs ready for single molecule measurements. Several alternative methods are described for each step, and ways of troubleshooting the most common problems, in particular, splinted RNA ligation, are suggested.
引用
收藏
页码：47 / 68
页数：22
相关论文
共 50 条
  • [31] Expanding the chemical scope of RNA:methyltransferases to site-specific alkynylation of RNA for click labeling
    Motorin, Yuri
    Burhenne, Juergen
    Teimer, Roman
    Koynov, Kaloian
    Willnow, Sophie
    Weinhold, Elmar
    Helm, Mark
    NUCLEIC ACIDS RESEARCH, 2011, 39 (05) : 1943 - 1952
  • [32] Enzymatic Site-Specific Labeling of RNA for Affinity Isolation of RNA-Protein Complexes
    Busby, Kayla N.
    Devaraj, Neal K.
    FASEB JOURNAL, 2018, 32 (01):
  • [33] Enzymatic site-specific labeling of RNA for affinity isolation of RNA-protein complexes
    Busby, Kayla
    Devaraj, Neal
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2018, 255
  • [34] Site-specific fluorescent labeling to visualize membrane translocation of a myristoyl switch protein
    Yang, Sung-Tae
    Lim, Sung In
    Kiessling, Volker
    Kwon, Inchan
    Tamm, Lukas K.
    SCIENTIFIC REPORTS, 2016, 6
  • [35] Site-specific fluorescent labeling to visualize membrane translocation of a myristoyl switch protein
    Sung-Tae Yang
    Sung In Lim
    Volker Kiessling
    Inchan Kwon
    Lukas K. Tamm
    Scientific Reports, 6
  • [36] Enhanced Site-Specific Fluorescent Labeling of Membrane Proteins Using Native Nanodiscs
    Ezsias, Bence
    Wolkenstein, Felix
    Goessweiner-Mohr, Nikolaus
    Yadav, Rohit
    Siligan, Christine
    Posch, Sandra
    Horner, Andreas
    Vargas, Carolyn
    Keller, Sandro
    Pohl, Peter
    BIOMOLECULES, 2025, 15 (02)
  • [37] Site-specific labeling of supercoiled DNA
    Lushnikov, Alexander Y.
    Potaman, Vladimir N.
    Lyubchenko, Yuri L.
    NUCLEIC ACIDS RESEARCH, 2006, 34 (16)
  • [38] Site-specific one-pot triple click labeling for DNA and RNA
    Winz, Marie-Luise
    Linder, Eva Christina
    Becker, Juliane
    Jaeschke, Andres
    CHEMICAL COMMUNICATIONS, 2018, 54 (83) : 11781 - 11784
  • [39] Site-specific fluorescein labeling of human insulin
    Liu, Fa
    Kohn, Wayne D.
    Mayer, John P.
    JOURNAL OF PEPTIDE SCIENCE, 2012, 18 (05) : 336 - 341
  • [40] SITE-SPECIFIC LABELING OF THE OLIGOSACCHARIDE CHAINS OF ANTIBODIES
    SCHWARZ, U
    WUNDERLICH, G
    BROSSMER, R
    FEBS LETTERS, 1994, 337 (02) : 213 - 216
12345