Determination of activity of denitrifying enzyme in soil samples by a headspace gas chromatographic technique

被引：2
|
作者：
Zhang, Xu-Bo [1 ]
Hu, Zhan-Bo [1 ]
Chai, Xin-Sheng [2 ]
Zhong, Guang-Zhi [3 ]
Ouyang, Xiao [1 ]
Li, Pei-Yi [1 ]
机构：
[1] Guangxi Univ, Sch Resources Environm & Mat, Nanning, Peoples R China
[2] South China Univ Technol, State Key Lab Pulp & Paper Engn, Guangzhou, Peoples R China
[3] Guangxi Beibu Gulf Investment Grp Co Ltd, Nanning, Peoples R China
来源：
JOURNAL OF CHROMATOGRAPHY A | 2021年 / 1638卷 / 1638期
关键词：
Headspace Gas Chromatography; Denitrifying enzyme; Nitrite nitrogen; Nitrogen dioxide;
D O I：
10.1016/j.chroma.2021.461882
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This paper reported a headspace gas chromatography (HS-GC) for the determination of denitrifying enzyme activity in soil samples. It was based on measuring the NO2 signal in a set of closed/air-free vials containing soil samples that incubated at the designated conditions. The results showed that the method has a good measurement precision (RSD < 5.0%) and reasonable accuracy for determining the denitrifying enzyme activity in soil samples. It is simple and efficient, and very suitable to be used in batch sample analysis. (C) 2021 Elsevier B.V. All rights reserved.
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页数：4
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