Subcritical fluid extraction of Stevia sweeteners from Stevia rebaudiana

被引:21
|
作者
Liu, J [1 ]
Ong, CP [1 ]
Li, SFY [1 ]
机构
[1] NATL UNIV SINGAPORE,DEPT CHEM,SINGAPORE 119260,SINGAPORE
关键词
D O I
10.1093/chromsci/35.9.446
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In this paper, the extraction of steviol glycosides in Stevia rebaudiana, including stevioside, rebaudioside A, rebaudioside C, and dulcoside A by subcritical fluid extraction (SubFE) is investigated. A simple, efficient SubFE method is developed. The extraction conditions (extraction phase composition, extraction time, etc.) are optimized. An extraction efficiency of more than 88% is obtained using methanol as a modifier. Determination of stevioside in the leaves of Stevia rebaudiana is performed by using the SubFE method developed and capillary electrophoresis.
引用
收藏
页码:446 / 450
页数:5
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