A Jurkat transcriptional reporter cell line for high-throughput analysis of the Nuclear Factor-κB signaling pathway

The transcription factor, Nuclear Factor-kappa B (NF-kappa B), regulates many genes involved in host immunity and cell survival. Unregulated NF-kappa B activity has been linked to many chronic inflammatory diseases and is an important target for the identification of inhibitors to better manage these disorders. We present a novel screening system to identify NF-kappa B inhibitors that combines sensitive fluorescence detection with medium- to high-throughput flow cytometry (HyperCyt (R)). To validate this approach, we quantified the activation of NF-kappa B by standard flow cytometry and the HyperCyt (R) platform. Results were comparable with regard to EC50 values for TNF alpha-mediated activation; however, the HyperCyt (R) platform provided more sensitive signal detection and a greater linear range for detection. To demonstrate the usefulness of this screening tool, we identified a novel inhibitor of NF-kappa B activation from a resveratrol-based chemical library. The inhibition of NF-kappa B activation by analog 6q (IC50 = 19 mu m) showed a 3.7-fold improvement over that of resveratrol (IC50 similar to 70 mu m).