Bioanalytical method validation of Esomeprazole by high performance liquid chromatography with PDA detection

被引:3
|
作者
Moni, Fatema [1 ]
Sharmin, Suriya [1 ]
Rony, Satyajit Roy [1 ]
Afroz, Farhana [1 ]
Akhter, Shammi [1 ]
Sohrab, Md Hossain [1 ]
机构
[1] Bangladesh CSIR BCSIR, Pharmaceut Sci Res Div, BCSIR Labs Dhaka, Dhaka 1205, Bangladesh
关键词
bioanalytical method validation; Esomeprazole; Pantoprazole; HPLC; PLASMA;
D O I
10.1556/1326.2020.00769
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
This study describes the development and validation of a simple, specific, accurate, and precise method for quantitative determination of Esomeprazole in human serum using Pantoprazole as internal standard (IS). After the addition of internal standard, Esomeprazole from serum samples was extracted simply by protein precipitation method followed by centrifugation and the supernatants were directly injected into the high performance liquid chromatography (HPLC). The chromatographic separation of the compounds was obtained on Hitachi Lachrom C8 column (5 mu m, 250 x 4.6 mm) with a mobile phase consisting of 5 mM potassium dihydrogen phosphate pH 7.4 and acetonitrile in a ratio of 70:30 with UV detection at 302 nm with a flow rate of 1 mL/min. The method was sensitive and specific, and validated over a concentration range of 0.06-6.0 mu g/mL. The limit of detection (LOD) and lower limit of quantification (LOQ) was 0.03 mu g/mL and 0.06 mu g/mL respectively. The precision and accuracy expressed as relative standard deviation were less than 15%. The average recovery of Esomeprazole from serum was 97.08%.
引用
收藏
页码:120 / 126
页数:7
相关论文
共 50 条
  • [41] DEVELOPMENT AND VALIDATION OF HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR THE DETERMINATION OF DARIFENACIN HYDROBROMIDE
    Divya, M.
    Padmalatha, H.
    INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2015, 2 (11): : 1525 - 1530
  • [42] Preliminary validation of high performance liquid chromatography method for detection of methyl-testosterone residue in carp muscle
    Jie Jiang
    Hong Lin
    Xiaoting Fu
    Mingming Li
    Journal of Ocean University of China, 2005, 4 (3) : 248 - 251
  • [43] Validation of a High Performance Liquid Chromatography Method for Aflatoxins Determination in Corn Arepas
    Martinez-Miranda, Marcela
    Taborda-Ocampo, Gonzalo
    Rosero-Moreano, Milton
    JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY, 2015, 26 (04) : 797 - 803
  • [44] Validation of a high performance liquid chromatography method for the determination of laminudine and zidovudine in tablets
    Beck, Ruy Carlos Ruver
    Cardoso, Simone Gon Alves
    Athayde, Margareth Linde
    Codevilla, Cristiane
    de Oliveira, Fernanda Kreutz
    Dalmora, Sergio Luiz
    QUIMICA NOVA, 2007, 30 (05): : 1225 - 1228
  • [45] Development and Validation of High Performance Liquid Chromatography Method for Determination Atorvastatin in Tablet
    Yugatama, A.
    Rohmani, S.
    Dewangga, A.
    INTERNATIONAL CONFERENCE ON ADVANCED MATERIALS FOR BETTER FUTURE 2017, 2018, 333
  • [46] Systematic Strategies in High-Performance Liquid Chromatography Method Development and Validation
    Shabir, Ghulam A.
    SEPARATION SCIENCE AND TECHNOLOGY, 2010, 45 (05) : 670 - 680
  • [47] Development and validation of the high performance liquid chromatography-ion exclusion method for detection of lactic acid in milk
    Milagres, Maria Patricia
    Cardoso Brandao, Sebastiao Cesar
    Magalhaes, Mirella Araujo
    Rodrigues Minim, Valeria Paula
    Minim, Luis Antonio
    FOOD CHEMISTRY, 2012, 135 (03) : 1078 - 1082
  • [48] Validation of a method of high performance liquid chromatography with fluorescence detection for melamine determination in UHT whole bovine milk
    Mendes Finete, Virginia de Lourdes
    Gouvea, Marcos Martins
    de Carvalho Marques, Flavia Ferreira
    Pereira Netto, Annibal Duarte
    FOOD CONTROL, 2015, 51 : 402 - 407
  • [49] Development and validation of an analytical method for the determination of ciprofloxacin in serum by High-Performance Liquid Chromatography with ultraviolet detection
    Falquez Manotas, Ana
    Sarmiento Rubiano, Luz
    ARCHIVOS DE MEDICINA, 2013, 13 (02): : 119 - 126