Galactosylation of human erythropoietin produced by chimeric chickens expressing galactosyltransferase

被引：9
|
作者：
Kojima, Yusuke [1 ]
Wakita, Jun [1 ]
Inayoshi, Yujin [1 ]
Suzuki, Ryosuke [1 ]
Yamada, Yuki [1 ]
Kaneoka, Hidenori [1 ]
Nishijima, Ken-ichi [1 ]
Iijima, Shinji [1 ]
机构：
[1] Nagoya Univ, Grad Sch Engn, Dept Biotechnol, Chikusa Ku, Nagoya, Aichi 4648603, Japan
来源：
JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2014年 / 117卷 / 06期
关键词：
Transgenic chicken; Erythropoietin; Galactose; Galactosyltransferase; N-Glycan; EGG-WHITE; SUGAR CHAINS; TRANSGENESIS; TRANSPORT; PROTEINS; ANTIBODY; YOLK;
D O I：
10.1016/j.jbiosc.2013.11.010
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Human erythropoietin produced in the egg white of chimeric chicken contains N-glycan with lower amounts of terminal galactose and sialic acid; therefore, the chicken galactosyltransferase gene was introduced together with the human erythropoietin gene by a retroviral vector. We found that erythropoietin accumulated in the egg white was partially galactosylated. (C) 2013, The Society for Biotechnology, Japan. All rights reserved.
引用
收藏
页码：676 / 679
页数：4
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