Adenoviral Transduction of Multipotent Mesenchymal Stromal Cells from Human Adipose Tissue with Bone Morphogenetic Protein BMP-2 Gene

被引：2
|
作者：
Bukharova, T. B. [1 ,2 ]
Logovskaya, L. V. [1 ,2 ]
Volkov, A. V. [2 ]
Garas, M. N. [3 ]
Vikhrova, E. B. [1 ,2 ]
Logunov, D. Yu. [3 ]
Makhnach, O. V. [1 ,2 ]
Shmarov, M. M. [3 ]
Gol'dshtein, D. V. [1 ,2 ]
机构：
[1] Russian Acad Med Sci, Moscow, Russia
[2] ReMeTeks Co, Moscow, Russia
[3] NF Gamalei Inst Epidemiol & Microbiol, Moscow, Russia
来源：
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2013年 / 156卷 / 01期
关键词：
multipotent mesenchymal stromal cells from adipose tissue; recombinant adenoviral particles; transduction; bone morphogenetic protein BMP-2; STEM-CELLS;
D O I：
10.1007/s10517-013-2294-y
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
We determined conditions for effective transduction of multipotent mesenchymal stromal cells from human adipose tissue with adenoviral constructs carrying the gene of human bone morphogenetic protein BMP-2. The peak of transgene transcription and BMP-2 protein secretion in the transduced cultures was observed on day 6 after infection. The maximum transcription of BMP-2 gene and genes of osteogenic markers (bone sialoprotein, osteopontin, and osteocalcin) was observed in the medium containing sodium beta-glycerophosphate and ascorbic acid. Addition of D-3 vitamin did not enhance the expression of BMP-2 gene in transduced cells. The obtained cell cultures with high osteogenic potential can be used in bone tissue engineering.
引用
收藏
页码：122 / 126
页数：5
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