Induction of Osteogenic Differentiation of Multipotent Mesenchymal Stromal Cells from Human Adipose Tissue

被引：7
|
作者：
Logovskaya, L. V. [1 ,2 ]
Bukharova, T. B. [1 ,2 ]
Volkov, A. V. [2 ]
Vikhrova, E. B. [1 ,2 ]
Makhnach, O. V. [1 ,2 ]
Goldshtein, D. V. [1 ,2 ]
机构：
[1] Russian Acad Med Sci, Med Genet Res Ctr, Moscow, Russia
[2] ReMeTeks Co, Moscow, Russia
来源：
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2013年 / 155卷 / 01期
关键词：
osteogenic differentiation; bone tissue regeneration; multipotent stromal adipose tissue cells; 1; alpha; 25-dihydroxycalciferol (vitamin D-3); dexamethasone; osteogenesis markers; DEXAMETHASONE;
D O I：
10.1007/s10517-013-2100-x
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
We studied of osteogenic differentiation of multipotent mesenchymal stromal cells from human adipose tissue. Experiments showed that 1 alpha,25-dihydroxycalciferol is a more effective inductor of osteogenesis than dexamethasone. Comparative analysis revealed activation of gene expression for the major osteogenic markers on day 7 of culturing in a medium containing 1 alpha,25-dihydroxycalciferol. It was found that transcription of genes encoding type 1 collagen proteins, osteopontin, osteocalcin, and bone sialoprotein peaked on day 14 in culture, while the expression of alkaline phosphatase and bone morphogenetic protein-2 genes increased over 21 days. Intensive mineralization of the extracellular matrix was observed starting from day 14 in culture. On the basis of the analysis of these data, optimal terms for osteogenic induction (day 14) and an optimal inductor (1 alpha,25-dihydroxycalciferol) were chosen and the protocol of effective osteogenic differentiation of multipotent mesenchymal stromal cells from human adipose tissue was developed for creation of tissue-engineered bone equivalents.
引用
收藏
页码：145 / 150
页数：6
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