Optimized ultrasonic-assisted extraction of polysaccharides from Cyclina sinensis and evaluation of antioxidant activities in vitro

被引:37
|
作者
Guo, Lei [1 ,2 ]
Zhu, Wencheng [1 ]
Xu, Fuquan [2 ]
Liu, Ming [1 ]
Xie, Yu [1 ]
Zhang, Jingmin [1 ]
机构
[1] Huaihai Inst Technol, Sch Ocean, Lianyungang 222005, Jiangsu, Peoples R China
[2] Huaihai Inst Technol, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Jiangsu, Peoples R China
关键词
Cyclina sinensis; polysaccharide; ultrasonic-assisted extraction; response surface methodology; antioxidant activity; RESPONSE-SURFACE METHODOLOGY; PLANT MATERIALS; PURIFICATION; COMPONENTS; CLAM; L;
D O I
10.1080/19476337.2013.785982
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The Box-Behnken design combined with response surface methodology was used to optimize ultrasonic-assisted extraction of polysaccharides from Cyclina sinensis. A high-extraction yield of polysaccharides, 34.631.52g/kg using an ultrasonic power of 500W, liquid to material ratio of 22:1 (mL/g), with an extraction temperature of 77 degrees C for 31min was achieved. The polysaccharides exhibited quite a strong reducing property, the total antioxidant capacity, as well as excellent inhibitions of DPPH radical, hydroxyl radical, and superoxide radical. Meanwhile, it was found that the bioactivities appeared to be dose-dependent on polysaccharides.
引用
收藏
页码:32 / 39
页数:8
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