hnRNP A2/B1 interacts with influenza A viral protein NS1 and inhibits virus replication potentially through suppressing NS1 RNA/protein levels and NS1 mRNA nuclear export

被引：30
|
作者：
Wang, Yimeng [1 ]
Zhou, Jianhong [1 ]
Du, Yuchun [1 ]
机构：
[1] Univ Arkansas, Dept Biol Sci, Fayetteville, AR 72701 USA
来源：
VIROLOGY | 2014年 / 449卷
基金：
美国国家卫生研究院;
关键词：
Influenza A virus; Proteomics; hnRNP A2/B1; NS1; Protein-protein interaction; mRNA nuclear export; BINDING-PROTEINS; NONSTRUCTURAL PROTEINS; POLYMERASE COMPLEX; PROTEOMIC APPROACH; A/B PROTEINS; EXPRESSION; TRANSLATION; CELLS; ACTIVATION; INTERFERON;
D O I：
10.1016/j.virol.2013.11.009
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The NS1 protein of influenza viruses is a major virulence factor and exerts its function through interacting with viral/cellular RNAs and proteins. In this study, we identified heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1) as an interacting partner of NS1 proteins by a proteomic method. Knockdown of hnRNP A2/B1 by small interfering RNA (siRNA) resulted in higher levels of NS vRNA, NS1 mRNA, and NS1 protein in the virus-infected cells. In addition, we demonstrated that hnRNP A2/B1 proteins are associated with NS1 and NS2 mRNAs and that knockdown of hnRNP A2/B1 promotes transport of NS1 mRNA from the nucleus to the cytoplasm in the infected cells. Lastly, we showed that knockdown of hnRNP A2/B1 leads to enhanced virus replication. Our results suggest that hnRNP A2/B1 plays an inhibitory role in the replication of influenza A virus in host cells potentially through suppressing NS1 RNA/protein levels and NS1 mRNA nucleocytoplasmic translocation. (C) 2013 Elsevier Inc. All rights reserved.
引用
收藏
页码：53 / 61
页数：9
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