Production of ethyl butyrate using gel-entrapped Candida cylindracea lipase

被引：43
|
作者：
Chen, JP
机构：
[1] Department of Chemical Engineering, Chang Gung Coll. Med./Technology, Kwei-San
来源：
JOURNAL OF FERMENTATION AND BIOENGINEERING | 1996年 / 82卷 / 04期
关键词：
lipase; ester synthesis; gel entrapment; prepolymer;
D O I：
10.1016/0922-338X(96)89160-4
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Candida cylindracea lipase was immobilized by entrapment in gels formed from photo-crosslinkable prepolymers for synthesis of ethyl butyrate by direct esterification of ethanol and butyric acid in hexane. Enzyme immobilization enhanced the enzyme activity up to 2.8 fold, improved the ester yield, and alleviated alcohol inhibition of the enzyme activity. The hydrophobic gel was better than a hydrophilic one in terms of overall performance, and the entrapped enzyme retained 70% of its initial activity after 15 cycles of batch reaction.
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页码：404 / 407
页数：4
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