Development and application of G1-ELISA for detection of antibodies against bovine ephemeral fever virus

被引：19

作者：

Zheng, Fu-ying ^{[1
]}

Lin, Guo-zhen ^{[1
]}

Qiu, Chang-qing ^{[1
]}

Zhou, Ji-zhang ^{[1
]}

Cao, Xiao-an ^{[1
]}

Gong, Xiao-wei ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Key Lab Grazing Anim Dis, State Key Lab Vet Etiol Biol, Key Lab Anim Virol,Lanzhou Vet Res Inst,Minist Ag, Lanzhou 730046, Gansu, Peoples R China

来源：

RESEARCH IN VETERINARY SCIENCE | 2009年 / 87卷 / 02期

关键词：

Bovine ephemeral fever virus; Indirect ELISA; MNT; Recombinant protein; INDIRECT ELISA; PROTEIN;

D O I：

10.1016/j.rvsc.2009.03.010

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The gene encoding antigenic site G(1) of bovine ephemeral fever virus (BEFV) was highly expressed in the host cell Escherichia coli. An indirect G(1)-ELISA with the recombinant protein as the coating antigen was established to detect antibodies against BEFV. The result revealed that the optimal concentration of the coated antigen was 0.5 mu g/well and the dilution of serum was 1:20. It was optimal that sera with P/N value >= 2.2 were considered positive, P/N value <= 2.0 negative, and between 2.0 and 2.2 ambiguous. The G(1)-ELISA method gave a sensitivity of 97.6% and a specificity of 98.6% by testing 590 field serum samples. These results suggest that the G(1)-ELISA may be a good alternative tool for seroepidemiological surveys. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：211 / 212

页数：2

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