New qualitative detection methods of genetically modified potatoes

被引:21
|
作者
Watanabe, T
Kuribara, H
Mishima, T
Kikuchi, H
Kubo, M
Kodama, T
Futo, S
Kasama, K
Toyota, A
Nouno, M
Saita, A
Takahashi, K
Hino, A
Akiyama, H
Maitani, T
机构
[1] Natl Inst Hlth Sci, Setagaya Ku, Tokyo 1588501, Japan
[2] Japan Food Res Labs, Chitose, Hokkaido 0660052, Japan
[3] FASMAC Co Ltd, Atsugi, Kanagawa 2430041, Japan
[4] Hatano Res Inst, Food & Drug Safety Ctr, Hiroshima 7340007, Japan
[5] Hiroshima Prefectural Inst Publ Hlth & Environm, Minami Ku, Hiroshima 7340007, Japan
[6] Ctr Inspect Imported Foods & Infect Dis, Yokohama Quarantine Stn, Kanazawa Ku, Yokohama, Kanagawa 2360011, Japan
[7] Natl Food Res Inst, Tsukuba, Ibaraki 3058642, Japan
关键词
genetically modified potato; detection method; NewLeaf; NewLeaf Plus; NewLeaf Y; uridine diphosphate (UDP) glucose; pyrophosphorylase (UGPase);
D O I
10.1248/bpb.27.1333
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
In Japan, 8 lines of genetically modified (GM) potato (2 lines of NewLeaf(R) potato; NL, 3 lines of NewLeaf Plus(R) potato; NLP, and 3 lines of NewLeaf Y(R) potato; NLY) have already been authorized as safe for use in foods and feeds. We have developed polymerase chain reaction (PCR) methods for the qualitative detection of the GM potatoes for the screening and the identification of NL, NLP and NLY. The gene encoding uridine diphosphate (UDP)-glucose pyrophosphorylase (UGPase) was used as a taxon specific gene. We designed the primer pair to detect the cryIIIA genes as a screening method for GM potatoes because the gene should be inserted in all 8 lines of the GM potatoes. For identification of NL, NLP and NLY, we further designed three specific primer pairs for the different recombinant DNAs (r-DNA) specifically introduced into NL, NLP, or NLY. In addition, to identify the 3 lines of NLY that have been introduced with the same r-DNA, the three line-specific primer pairs for the border sequence between the r-DNA and genomic DNA of NLY 3 lines were designed. Six lines of GM potato used as the test material were specifically identified using the each primer pair under the same PCR condition. The detection limits of all the GM potatoes should be approximately 0.1%. Furthermore, the specificity and reproducibility of the methods were confirmed in a six-laboratory collaborative study.
引用
收藏
页码:1333 / 1339
页数:7
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