Translation initiation factor eIF4G mediates in vitro poly(A) tail-dependent translation

被引：270

作者：

Tarun, SZ ^{[1
]}

Wells, SE ^{[1
]}

Deardorff, JA ^{[1
]}

Sachs, AB ^{[1
]}

机构：

[1] UNIV CALIF BERKELEY,DEPT MOL & CELL BIOL,BERKELEY,CA 94720

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1997年 / 94卷 / 17期

关键词：

D O I：

10.1073/pnas.94.17.9046

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The yeast translation factor eIF4G associates with both the cap-binding protein eIF4E and the poly(A)-binding protein Pab1p. Here we report that the two yeast eIF4G homologs, Tif4631p and Tif4632p, share a conserved Pab1p-binding site. This site is required for Pab1p and poly(A) tails to stimulate the in vitro translation of uncapped polyadenylylated mRNA, and the region encompassing it is required for the cap and the poly(A) tail to synergistically stimulate translation, This region on Tif4631p becomes essential for cell growth when the eIF4E binding site on Tif4631p is mutated. Pab1p mutations also show synthetic lethal interactions with eIF4E mutations, These data suggest that eIF4G mediates poly(A) tail stimulated translation in vitro, and that Pab1p and the domain encompassing the Pab1p-binding site an eIF4G can compensate for partial loss of eIF4E function in vivo.

引用

页码：9046 / 9051

页数：6

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