Polarized distribution of NHE1 and NHE2 in the rat epididymis

被引：29
|
作者：
Chew, SBC
Leung, GPH
Leung, PY
Tse, CM
Wong, PYD [1 ]
机构：
[1] Chinese Univ Hong Kong, Dept Physiol, Shatin, Hong Kong, Peoples R China
[2] Chinese Univ Hong Kong, Fac Med, Shatin, Hong Kong, Peoples R China
[3] Johns Hopkins Univ Hosp, Sch Med, Div Gastroenterol, Dept Med, Baltimore, MD 21205 USA
来源：
BIOLOGY OF REPRODUCTION | 2000年 / 62卷 / 03期
关键词：
D O I：
10.1095/biolreprod62.3.755
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Previous studies from our laboratory have provided evidence that the rat epididymis utilizes the Na+/H+ exchanger to transport acid and base. The present study was undertaken to use immunohistochemistry for investigating the localization (apical versus basolateral) and distribution of NHE1 and NHE2 proteins along intact rat epididymis. Both proteins were found to be exclusively localized within the epithelium. Immunoreactivity for NHE1 was detected on the basolateral surface, whereas NHE2 immunoreactivity was detected on the apical side of the epithelium. Interestingly, NHE1 was found along the entire length of the epididymal tubule whereas NHE2 was absent in the initial segment but present in the caput, corpus, and cauda regions. These results, when interpreted along with those of previous functional studies, may suggest that the apical NHE2 is involved in Na+ reabsorption and the basolateral NHE1 in HCO3- secretion in the rat epididymis.
引用
收藏
页码：755 / 758
页数：4
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