Liquid chromatography tandem mass spectrometry method for the quantitation of NTBC (2-(nitro-4-trifluoromethylbenzoyl)1,3-cyclohexanedione) in plasma of tyrosinemia type 1 patients

被引:21
|
作者
Herebian, Diran [1 ]
Spiekerkoetter, Ute [1 ]
Lamshoeft, Marc [2 ]
Thimm, Eva [1 ]
Laryea, Maurice [1 ]
Mayatepek, Ertan [1 ]
机构
[1] Univ Dusseldorf, Univ Childrens Hosp, Dept Gen Paediat, D-4000 Dusseldorf, Germany
[2] Univ Dortmund, Inst Environm Res, D-44221 Dortmund, Germany
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2009年 / 877卷 / 14-15期
关键词
NTBC; Tyrosinemia type 1; HPLC-ESI-MS/MS; LTQ-Orbitrap; INHIBITORS;
D O I
10.1016/j.jchromb.2009.03.014
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In this study, we describe a bioanalytical method for quantification of NTBC in plasma of patients with hereditary tyrosinemia type 1 (HT-1) using high-performance liquid chromatography Coupled to tandem mass spectrometry (LC-MS/MS). After protein precipitation with acetonitrile including Mesotrione as internal standard, separation of NTBC was achieved by RP-HPLC. Detection was performed by positive ion electrospray ionization (ESI) in selected reaction monitoring (SRM) mode. NTBC recovery in the developed method was found to be more than 90%. The lower limit Of quantification was calculated to be 0.35 mu M. The intra-day and inter-clay precision of three different quality control samples (measured as RSD%) was less than 10% and 15%, respectively. The standard calibration curves showed good linearity within the range of 2.5-40 mu M and the determined correlation coefficients were r(2) >= 0.995. This presented method is rapid. sensitive, specific and suitable for clinical practice and research. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:1453 / 1459
页数:7
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