A reverse-phase HPLC assay for the simultaneous determination of enrofloxacin and ciprofloxacin in pig faeces

被引:23
|
作者
Sunderland, J
Lovering, AM
Tobin, CM
MacGowan, AP
Roe, JM
Delsol, AA
机构
[1] Southmead Gen Hosp, N Bristol NHS Trust, Bristol Ctr Antimicrobial Res & Evaluat, Dept Microbiol, Bristol BS10 5NB, Avon, England
[2] Univ Bristol, Div Farm Anim Sci, Dept Vet Clin Sci, Bristol BS40 5DU, Avon, England
关键词
enrofloxacin; ciprofloxacim; metabolite; pig faeces;
D O I
10.1016/j.ijantimicag.2003.07.014
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
A reverse-phase HPLC assay is described for the simultaneous assay of enrofloxacin (ENR) and ciprofloxacin (CPX) in pig faeces. Extraction used dichloromethane, 2-propanol and 0.3 M ortho-phosphoric acid (1:5:4 v/v/v). Separation was achieved using a Spherisorb S5 C8 column, heated to 50degreesC and a mobile phase of 0.16% ortho-phosphoric acid (adjusted to pH 3.0 with tetrabutylammonium hydroxide solution) with 20 ml acetonitrile per litre solution. The method used fluorescence detection (Ex 3 10 nm Ern 445 nm), a flow rate of 1 ml/min and a 20 mul injection volume. Retention times were approximately 6 min for ciprofloxacin and 10min for enrofloxacin. The linearity range for both compounds was 0-20 mg/kg, lowest limit of quantification 0.3 mg/kg and recoveries were >92%. (C) 2003 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
引用
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页码:390 / 393
页数:4
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