Production of penicillin G amidase from Alcaligenes faecalis in a recombinant Escherichia coli

被引：0
|
作者：
Gou, Bin-Quan [1 ]
Chu, Ju [1 ]
Zhang, Si-Liang [1 ]
Wang, Yong-Hong [1 ]
Zhuang, Ying-Ping [1 ]
Huang, Hua [1 ]
Li, Zhen [1 ]
Yuan, Zhong-Yi [1 ]
机构：
[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
来源：
JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS | 2009年 / 40卷 / 02期
关键词：
Alcaligenes faecalis; Constitutive expression; Fed-batch fermentation; Penicillin G amidase; Recombinant E. coli; G ACYLASE; GENE;
D O I：
10.1016/j.jtice.2008.09.004
中图分类号：
TQ [化学工业];
学科分类号：
0817 ;
摘要：
Penicillin G amidase (PGA) from Alcaligenes faecalis, which has useful properties for combinatorial biochemistry, is produced by a recombinant Escherichia coli using a constitutive expression system. Cells are cultured on a semi-defined medium in a 5-L fermentor using fed-batch technology. The fermentation yield is about 23,000 U PGA activity/L broth. (C) 2008 Taiwan Institute of Chemical Engineers. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：233 / 236
页数：4
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