Simultaneous identification of species and molecular forms of the Anopheles gambiae complex by PCR-RFLP

被引：385
|
作者：
Fanello, C
Santolamazza, F
della Torre, A
机构：
[1] Univ Roma La Sapienza, Dipartimento Sci Sanita Publ, Sez Parassitol, I-00185 Rome, Italy
[2] London Sch Hyg & Trop Med, London WC1, England
来源：
MEDICAL AND VETERINARY ENTOMOLOGY | 2002年 / 16卷 / 04期
关键词：
Anopheles gambiae complex; malaria vectors; molecular markers; mosquito identification; PCR-RFLP; sibling species; Africa;
D O I：
10.1046/j.1365-2915.2002.00393.x
中图分类号：
Q96 [昆虫学];
学科分类号：
摘要：
For differential identification of sibling species in the Anopheles gambiae Giles complex (Diptera: Culicidae), including simultaneous separation of M and S molecular forms within An. gambiae Giles sensu stricto , we describe a PCR-RFLP method. This procedure is more efficient, faster and cheaper than those used before, so is recommended for large-scale processing of field-collected larval and adult specimens to be identified in malaria vector studies.
引用
收藏
页码：461 / 464
页数：4
相关论文
共 50 条
  • [1] Simultaneous identification of molecular and mating types within the Cryptococcus species complex by PCR-RFLP analysis
    Feng, Xiaobo
    Yao, Zhirong
    Ren, Daming
    Liao, Wanqing
    [J]. JOURNAL OF MEDICAL MICROBIOLOGY, 2008, 57 (12) : 1481 - 1490
  • [2] Simultaneous identification of the Anopheles funestus group and Anopheles longipalpis type C by PCR-RFLP
    Kwang Shik Choi
    Maureen Coetzee
    Lizette L Koekemoer
    [J]. Malaria Journal, 9
  • [3] Simultaneous identification of the Anopheles funestus group and Anopheles longipalpis type C by PCR-RFLP
    Choi, Kwang Shik
    Coetzee, Maureen
    Koekemoer, Lizette L.
    [J]. MALARIA JOURNAL, 2010, 9
  • [4] Molecular identification of weedy Sporobolus species by PCR-RFLP
    Shrestha, S.
    Graham, G. C.
    Loch, D. S.
    Adkins, S. W.
    [J]. WEED RESEARCH, 2010, 50 (05) : 383 - 387
  • [5] Identification of two cryptic species in the Anopheles (Cellia) annularis complex using ribosomal DNA PCR-RFLP
    Alam, Mohammad Tauqeer
    Das, Manoj K.
    Dev, Vas
    Ansari, Musharraf A.
    Sharma, Yagya D.
    [J]. PARASITOLOGY RESEARCH, 2007, 100 (05) : 943 - 948
  • [6] Identification of two cryptic species in the Anopheles (Cellia) annularis complex using ribosomal DNA PCR-RFLP
    Mohammad Tauqeer Alam
    Manoj K. Das
    Vas Dev
    Musharraf A. Ansari
    Yagya D. Sharma
    [J]. Parasitology Research, 2007, 100 : 943 - 948
  • [7] PCR-RFLP assays for the identification of Anopheles (Diptera: Culicidae) species circulating in Honduras
    Escobar, Denis
    Perez, Fernando
    Ortiz, Bryan
    Fontecha, Gustavo
    [J]. MALARIA JOURNAL, 2023, 22 (01)
  • [8] Molecular identification of grouper species using PCR-RFLP technique
    Sumathi, G.
    Jeyasekaran, G.
    Shakila, R. Jeya
    Sivaraman, B.
    Arunkumar, G.
    Manimaran, U.
    Sukumar, D.
    [J]. FOOD CONTROL, 2015, 51 : 300 - 306
  • [9] Molecular identification of ovine Theileria species by a new PCR-RFLP method
    Bami, M. Heidarpour
    Haddadzadeh, H. R.
    Kazemi, B.
    Khazraiinia, P.
    Bandehpour, M.
    Aktas, M.
    [J]. VETERINARY PARASITOLOGY, 2009, 161 (3-4) : 171 - 177
  • [10] Identification of common species of dermatophytes by PCR-RFLP
    He Ganlin
    Li Jiawen
    Ding Juan
    Tan Zhijan
    [J]. Journal of Huazhong University of Science and Technology [Medical Sciences], 2005, 25 (4): : 458 - 460
12345