Simultaneous identification of species and molecular forms of the Anopheles gambiae complex by PCR-RFLP

被引：389
|
作者：
Fanello, C
Santolamazza, F
della Torre, A
机构：
[1] Univ Roma La Sapienza, Dipartimento Sci Sanita Publ, Sez Parassitol, I-00185 Rome, Italy
[2] London Sch Hyg & Trop Med, London WC1, England
来源：
MEDICAL AND VETERINARY ENTOMOLOGY | 2002年 / 16卷 / 04期
关键词：
Anopheles gambiae complex; malaria vectors; molecular markers; mosquito identification; PCR-RFLP; sibling species; Africa;
D O I：
10.1046/j.1365-2915.2002.00393.x
中图分类号：
Q96 [昆虫学];
学科分类号：
摘要：
For differential identification of sibling species in the Anopheles gambiae Giles complex (Diptera: Culicidae), including simultaneous separation of M and S molecular forms within An. gambiae Giles sensu stricto , we describe a PCR-RFLP method. This procedure is more efficient, faster and cheaper than those used before, so is recommended for large-scale processing of field-collected larval and adult specimens to be identified in malaria vector studies.
引用
收藏
页码：461 / 464
页数：4
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