Molecular cloning of growth hormone encoding cDNA of Indian major carps by a modified rapid amplification of cDNA ends strategy

被引：4
|
作者：
Venugopal, T [1 ]
Mathavan, S [1 ]
Pandian, TJ [1 ]
机构：
[1] Madurai Kamaraj Univ, Sch Biol Sci, Ctr Adv Studies Funct Genom, Madurai 625021, Tamil Nadu, India
来源：
JOURNAL OF BIOSCIENCES | 2002年 / 27卷 / 03期
关键词：
cDNA cloning; growth hormone; Indian major carps; overexpression; protein 3-D structure; RACE;
D O I：
10.1007/BF02704915
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A modified rapid amplification of cDNA ends (RACE) strategy has been developed for cloning highly conserved cDNA sequences. Using this modified method, the growth hormone (GH) encoding cDNA sequences of Labeo rohita, Cirrhina mrigala and Catla catla have been cloned, characterized and overexpressed in Escherichia coli. These sequences show 96-98% homology to each other and are about 85% homologous to that of common carp. Besides, an attempt has been made for the first time to describe a 3-D model of the fish GH protein.
引用
收藏
页码：261 / 272
页数：12
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