Stable and efficient immobilization of bi-enzymatic NADPH cofactor recycling system under consecutive microwave irradiation

被引:10
|
作者
Chen, Rong [1 ,2 ,3 ]
Wei, Qiuhui [1 ]
Wei, Xin [1 ]
Liu, Yuheng [1 ]
Zhang, Xiaomin [1 ]
Chen, Xiabin [1 ]
Yin, Xiaopu [1 ]
Xie, Tian [1 ]
机构
[1] Hangzhou Normal Univ, Key Lab Elemene Class Anticanc Chinese Med Zhejia, Engn Lab Dev & Applicat Tradit Chinese Med Zhejia, Holist Integrat Pharm Inst,Sch Med, Hangzhou, Peoples R China
[2] Hangzhou Normal Univ, Fac Prevent Med, Hangzhou, Peoples R China
[3] Hangzhou Normal Univ, Coll Med, Hangzhou, Peoples R China
来源
PLOS ONE | 2020年 / 15卷 / 11期
基金
中国国家自然科学基金;
关键词
COVALENT IMMOBILIZATION; REGENERATION; REDUCTASE;
D O I
10.1371/journal.pone.0242564
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
One of the challenges in biocatalysis is the development of stable and efficient bi-enzymatic cascades for bio-redox reactions coupled to the recycling of soluble cofactors. Aldo-keto reductase (LEK) and glucose dehydrogenase (GDH) can be utilized as the NADPH recycling system for economic and efficient biocatalysis of (R)-4-chloro-3-hydroxybutanoate ((R)-CHBE), an important chiral pharmaceutical intermediate. The LEK and GDH was efficiently co-immobilized in mesocellular siliceous foams (MCFs) under microwave irradiation (CoLG-MIA). while they were also co-immobilized by entrapment in calcium alginate without MIA as control (CoLG-CA). The relative activity of CoLG-MIA was increased to 140% compared with that of free LEK. The CoLG-MIA exhibited a wider range of pH and temperature stabilities compared with other preparations. The thermal, storage and batch operational stabilities of microwave-assisted immobilized LEK-GDH were also improved. The NADPH recycling system exhibited the potential as the stable and efficient catalyst for the industrial preparation of (R)-CHBE.
引用
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页数:15
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