Stable and efficient immobilization of bi-enzymatic NADPH cofactor recycling system under consecutive microwave irradiation

被引:10
|
作者
Chen, Rong [1 ,2 ,3 ]
Wei, Qiuhui [1 ]
Wei, Xin [1 ]
Liu, Yuheng [1 ]
Zhang, Xiaomin [1 ]
Chen, Xiabin [1 ]
Yin, Xiaopu [1 ]
Xie, Tian [1 ]
机构
[1] Hangzhou Normal Univ, Key Lab Elemene Class Anticanc Chinese Med Zhejia, Engn Lab Dev & Applicat Tradit Chinese Med Zhejia, Holist Integrat Pharm Inst,Sch Med, Hangzhou, Peoples R China
[2] Hangzhou Normal Univ, Fac Prevent Med, Hangzhou, Peoples R China
[3] Hangzhou Normal Univ, Coll Med, Hangzhou, Peoples R China
来源
PLOS ONE | 2020年 / 15卷 / 11期
基金
中国国家自然科学基金;
关键词
COVALENT IMMOBILIZATION; REGENERATION; REDUCTASE;
D O I
10.1371/journal.pone.0242564
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
One of the challenges in biocatalysis is the development of stable and efficient bi-enzymatic cascades for bio-redox reactions coupled to the recycling of soluble cofactors. Aldo-keto reductase (LEK) and glucose dehydrogenase (GDH) can be utilized as the NADPH recycling system for economic and efficient biocatalysis of (R)-4-chloro-3-hydroxybutanoate ((R)-CHBE), an important chiral pharmaceutical intermediate. The LEK and GDH was efficiently co-immobilized in mesocellular siliceous foams (MCFs) under microwave irradiation (CoLG-MIA). while they were also co-immobilized by entrapment in calcium alginate without MIA as control (CoLG-CA). The relative activity of CoLG-MIA was increased to 140% compared with that of free LEK. The CoLG-MIA exhibited a wider range of pH and temperature stabilities compared with other preparations. The thermal, storage and batch operational stabilities of microwave-assisted immobilized LEK-GDH were also improved. The NADPH recycling system exhibited the potential as the stable and efficient catalyst for the industrial preparation of (R)-CHBE.
引用
收藏
页数:15
相关论文
共 23 条
  • [1] Stable and efficient immobilization technique of aldolase under consecutive microwave irradiation at low temperature
    Wang, Anming
    Wang, Meizhang
    Wang, Qiuyan
    Chen, Feifei
    Zhang, Fangkai
    Li, Haifeng
    Zeng, Zhaowu
    Xie, Tian
    BIORESOURCE TECHNOLOGY, 2011, 102 (02) : 469 - 474
  • [2] A bi-enzymatic system for efficient enantioselective bioconversion of racemic mandelic acid
    Wang, Peng
    Yang, Junfang
    Jiang, Lei
    Feng, Jing
    Yang, Chengli
    Li, Dali
    JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2013, 94 : 47 - 50
  • [3] Kinetic Modeling of a Bi-Enzymatic System for Efficient Conversion of Lactose to Lactobionic Acid
    Van Hecke, Wouter
    Bhagwat, Aditya
    Ludwig, Rolaud
    Dewulf, Jo
    Haltrich, Dietmar
    Van Langehove, Herman
    BIOTECHNOLOGY AND BIOENGINEERING, 2009, 102 (05) : 1475 - 1482
  • [4] Bi-enzymatic nanobiocatalyst based on immobilization of xylanase and pectinase onto functionalized magnetic nanoparticles for efficient fruit juice clarification
    Kharazmi, Sara
    Taheri-Kafrani, Asghar
    LWT-FOOD SCIENCE AND TECHNOLOGY, 2023, 183
  • [5] Co-immobilization of a bi-enzymatic cascade into hierarchically porous MIL-53 for efficient 6′-sialyllactose production
    Peng, Xinlv
    Pei, Caixia
    Qian, Eika W.
    Du, Yuguang
    Li, Jian-Jun
    NANOSCALE, 2024, 16 (31) : 14932 - 14939
  • [6] High efficient and covalent immobilization of penicillin acylase under microwave irradiation
    Wang Hua
    Wang An-Ming
    Zhou Cheng
    Zong She-Min
    Zong Jing
    Shen Shu-Bao
    ACTA CHIMICA SINICA, 2008, 66 (02) : 271 - 275
  • [7] A novel and efficient method for the immobilization of thermolysin using sodium chloride salting-in and consecutive microwave irradiation
    Chen, Feifei
    Zhang, Fangkai
    Du, Fangchuan
    Wang, Anming
    Gao, Weifang
    Wang, Qiuyan
    Yin, Xiaopu
    Xie, Tian
    BIORESOURCE TECHNOLOGY, 2012, 115 : 158 - 163
  • [8] Development of a novel bi-enzymatic silver dendritic hierarchical nanostructure cascade catalytic system for efficient conversion of starch into gluconic acid
    Rezaei, Saghar
    Landarani-Isfahani, Amir
    Moghadam, Majid
    Tangestaninejad, Shahram
    Mirkhani, Valiollah
    Mohammadpoor-Baltork, Iraj
    CHEMICAL ENGINEERING JOURNAL, 2019, 356 : 423 - 435
  • [9] Zeofen, an efficient reagent for oxidative deprotection of trimethylsilyl ethers under microwave irradiation in solventless system
    Heravi, MM
    Ajami, D
    Ghassemzadeh, M
    Tabar-Hydar, K
    SYNTHETIC COMMUNICATIONS, 2001, 31 (14) : 2097 - 2100
  • [10] Nanostructured N doped TiO2efficient stable catalyst for Kabachnik-Fields reaction under microwave irradiation
    Kunde, Sachin P.
    Kanade, Kaluram G.
    Karale, Bhausaheb K.
    Akolkar, Hemant N.
    Arbuj, Sudhir S.
    Randhavane, Pratibha, V
    Shinde, Santosh T.
    Shaikh, Mubarak H.
    Kulkarni, Aniruddha K.
    RSC ADVANCES, 2020, 10 (45) : 26997 - 27005