共 23 条
Stable and efficient immobilization of bi-enzymatic NADPH cofactor recycling system under consecutive microwave irradiation
被引:10
|作者:
Chen, Rong
[1
,2
,3
]
Wei, Qiuhui
[1
]
Wei, Xin
[1
]
Liu, Yuheng
[1
]
Zhang, Xiaomin
[1
]
Chen, Xiabin
[1
]
Yin, Xiaopu
[1
]
Xie, Tian
[1
]
机构:
[1] Hangzhou Normal Univ, Key Lab Elemene Class Anticanc Chinese Med Zhejia, Engn Lab Dev & Applicat Tradit Chinese Med Zhejia, Holist Integrat Pharm Inst,Sch Med, Hangzhou, Peoples R China
[2] Hangzhou Normal Univ, Fac Prevent Med, Hangzhou, Peoples R China
[3] Hangzhou Normal Univ, Coll Med, Hangzhou, Peoples R China
来源:
基金:
中国国家自然科学基金;
关键词:
COVALENT IMMOBILIZATION;
REGENERATION;
REDUCTASE;
D O I:
10.1371/journal.pone.0242564
中图分类号:
O [数理科学和化学];
P [天文学、地球科学];
Q [生物科学];
N [自然科学总论];
学科分类号:
07 ;
0710 ;
09 ;
摘要:
One of the challenges in biocatalysis is the development of stable and efficient bi-enzymatic cascades for bio-redox reactions coupled to the recycling of soluble cofactors. Aldo-keto reductase (LEK) and glucose dehydrogenase (GDH) can be utilized as the NADPH recycling system for economic and efficient biocatalysis of (R)-4-chloro-3-hydroxybutanoate ((R)-CHBE), an important chiral pharmaceutical intermediate. The LEK and GDH was efficiently co-immobilized in mesocellular siliceous foams (MCFs) under microwave irradiation (CoLG-MIA). while they were also co-immobilized by entrapment in calcium alginate without MIA as control (CoLG-CA). The relative activity of CoLG-MIA was increased to 140% compared with that of free LEK. The CoLG-MIA exhibited a wider range of pH and temperature stabilities compared with other preparations. The thermal, storage and batch operational stabilities of microwave-assisted immobilized LEK-GDH were also improved. The NADPH recycling system exhibited the potential as the stable and efficient catalyst for the industrial preparation of (R)-CHBE.
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页数:15
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