Immobilization of Lipase by Adsorption Onto Magnetic Nanoparticles in Organic Solvents

被引：21

作者：

Shi, Ying ^{[1
]}

Liu, Wei ^{[1
]}

Tao, Qing-Lan ^{[1
]}

Jiang, Xiao-Ping ^{[1
]}

Liu, Cai-Hong ^{[1
]}

Zeng, Sha ^{[1
]}

Zhang, Ye-Wang ^{[1
]}

机构：

[1] Jiangsu Univ, Sch Pharm, Zhenjiang 212013, Peoples R China

来源：

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2016年 / 16卷 / 01期

基金：

美国国家科学基金会;

关键词：

Lipase; Organic Solvent; Immobilization; Thermostability; Magnetic Nanoparticles; CATALYZED REACTIONS; WATER SAMPLES; STABILITY; MICROSPHERES; FABRICATION; EXTRACTION; MEDIA;

D O I：

10.1166/jnn.2016.10694

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In order to improve the performance of lipase in organic solvents, a simple immobilization method was developed by adsorption of lipase onto Fe3O4@SiO2 magnetic nanoparticles in organic solvent. Among the solvents tested, toluene was found to be the most effective solvent for the immobilization. A maximum immobilization yield of 97% and relative activity of 124% were achieved in toluene at 30 degrees C. The optimal temperature, enzyme loading and water activity were 30 degrees C, 1.25 mg/mg support and 0.48 aw, respectively. The residual activity of immobilized lipase was 67% after 10 cycles of use. The advantages of the immobilized lipase including easy recovery, high stability, and enhanced activity of immobilized lipase in organic solvents show potential industrial applications in anhydrous solvents.

引用

页码：601 / 607

页数：7

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