Analysis of the hemolymph proteins from the acorn barnacle Megabalanus rosa by two-dimensional gel electrophoresis

被引：2
|
作者：
Muramoto, K
Kuriki, T
Nokihara, K
Yako, H
Kamiya, H
机构：
[1] SHIMADZU CO LTD,DEPT BIOTECHNOL INSTRUMENTS,KYOTO 604,JAPAN
[2] TOKYO UNIV AGR & TECHNOL,KOGANEI,TOKYO 184,JAPAN
[3] KITASATO UNIV,SCH FISHERIES SCI,SANRIKU,IWATE 02201,JAPAN
来源：
FISHERIES SCIENCE | 1996年 / 62卷 / 03期
关键词：
hemolymph protein; lectins; barnacle; two-dimensional gel electrophoresis; amino acid sequence;
D O I：
10.2331/fishsci.62.435
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
The hemolymph proteins of the acorn barnacle Megabalanus rosa were separated by two-dimensional gel electrophoresis. More than 50 proteins were observed in the region of acidic (pl: 4-7) with masses of 18-120 kDa. The proteins separated on the gel were electroblotted onto a polyvinylidene difluoride membrane and were sequenced. Several major proteins were further characterized by amino acid analysis and peptide mapping, and identified to be multiple lectins.
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页码：435 / 438
页数：4
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