DEVELOPMENT OF A METHOD FOR DETERMINATION OF TIOCONAZOLE ASSOCIATED WITH POLYMERIC NANOCAPSULES BY LIQUID CHROMATOGRAPHY.

被引：5
|
作者：
Haerter, Andreia P. G. [1 ]
Motta, Mariana H. [1 ]
de Barros, Julia G. [1 ]
Ribeiro, Roseane F. [1 ]
Adams, Andrea I. H. [1 ]
Schaffazick, Scheila R. [1 ]
da Silva, Cristiane de B. [1 ]
机构：
[1] Univ Fed Santa Maria, Dept Farm Ind, Cr Ciencias Saude, BR-97105900 Santa Maria, RS, Brazil
来源：
QUIMICA NOVA | 2014年 / 37卷 / 06期
关键词：
polymeric nanocapsules; tioconazole; validation; RP-HPLC METHOD; VALIDATION; DRUGS;
D O I：
10.5935/0100-4042.20140153
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The aim of this work was to develop and validate an analytical method for the quantification of tioconazole in polymeric nanocapsule suspensions by high performance liquid chromatography with UV detection. The analysis was performed with a mobile phase composed of methanol: water (80: 20) and 0.18% ammonium hydroxide; RP-18 column and UV detection at 219 nm. The method proved to be linear in the concentration range of 5-50 mu g mL(-1) (r = 0.9999), specific, precise (repeatability RSD = 1.42%, intermediate precision RSD = 1.17%), accurate (98 - 102%) and robust (RSD < 2.0%). In conclusion, a simple and rapid method was validated proving suitable for quantification of tioconazole in polymeric nanocapsules.
引用
收藏
页码：996 / 999
页数：4
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