DEVELOPMENT OF A METHOD FOR DETERMINATION OF TIOCONAZOLE ASSOCIATED WITH POLYMERIC NANOCAPSULES BY LIQUID CHROMATOGRAPHY.

被引：5
|
作者：
Haerter, Andreia P. G. [1 ]
Motta, Mariana H. [1 ]
de Barros, Julia G. [1 ]
Ribeiro, Roseane F. [1 ]
Adams, Andrea I. H. [1 ]
Schaffazick, Scheila R. [1 ]
da Silva, Cristiane de B. [1 ]
机构：
[1] Univ Fed Santa Maria, Dept Farm Ind, Cr Ciencias Saude, BR-97105900 Santa Maria, RS, Brazil
来源：
QUIMICA NOVA | 2014年 / 37卷 / 06期
关键词：
polymeric nanocapsules; tioconazole; validation; RP-HPLC METHOD; VALIDATION; DRUGS;
D O I：
10.5935/0100-4042.20140153
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The aim of this work was to develop and validate an analytical method for the quantification of tioconazole in polymeric nanocapsule suspensions by high performance liquid chromatography with UV detection. The analysis was performed with a mobile phase composed of methanol: water (80: 20) and 0.18% ammonium hydroxide; RP-18 column and UV detection at 219 nm. The method proved to be linear in the concentration range of 5-50 mu g mL(-1) (r = 0.9999), specific, precise (repeatability RSD = 1.42%, intermediate precision RSD = 1.17%), accurate (98 - 102%) and robust (RSD < 2.0%). In conclusion, a simple and rapid method was validated proving suitable for quantification of tioconazole in polymeric nanocapsules.
引用
收藏
页码：996 / 999
页数：4
相关论文
共 50 条
  • [1] Development of an ultraperformance liquid chromatography method for improved determination of additives in polymeric materials
    Noguerol-Cal, Rosalia
    Lopez-Vilarino, Jose Manuel
    Gonzalez-Rodriguez, M. Victoria
    Barral-Losada, Luis F.
    [J]. JOURNAL OF SEPARATION SCIENCE, 2007, 30 (15) : 2452 - 2459
  • [2] Determination of submicromolar concentrations of formaldehyde by liquid chromatography.
    Jones, SB
    Terry, CM
    Lister, TE
    Johnson, DC
    Gardner, DL
    [J]. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2000, 219 : U90 - U90
  • [3] Determination of lactose by high performance liquid chromatography.
    Sexton, D
    Ho, CN
    [J]. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2004, 228 : U126 - U126
  • [4] DETERMINATION OF PESTICIDES BY GAS CHROMATOGRAPHY AND HIGH PRESSURE LIQUID CHROMATOGRAPHY.
    Eagle, David J.
    Jones, John L.O.
    Jewell, Edward J.
    [J]. 1983, : 455 - 511
  • [5] Determination of plasma glutamine by high performance liquid chromatography.
    Liu, FN
    Sheng, XQ
    Chen, YM
    [J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, 1996, 23 (05) : 475 - 477
  • [6] DETERMINATION OF ASCORBYL PALMITATE BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY.
    Vicente, Thelma S.
    Waysek, Edward H.
    Cort, Winifred M.
    [J]. JAOCS, Journal of the American Oil Chemists' Society, 1985, 62 (04): : 745 - 747
  • [7] Determination of progesterone in nanocapsules by high performance liquid chromatography
    Benali, S
    TharasseBloch, C
    Andre, D
    Verite, P
    Duclos, R
    Lafont, O
    [J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 1997, 20 (19) : 3233 - 3243
  • [8] Validation of high performance liquid chromatography method for determination of meloxicam loaded PEGylated nanocapsules
    Ianiski, Francine Rodrigues
    Laporta, Luciane Varini
    Rubim, Alexandre Machado
    Luchese, Cristiane
    [J]. BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2015, 51 (04) : 823 - 832
  • [9] Thorsten Teutenberg: High Temperature Liquid Chromatography. A User’s Guide for Method Development
    Tony Edge
    [J]. Chromatographia, 2011, 74 (5-6) : 509 - 510
  • [10] Determination of carotenoids in Jiao-Gu-Lan by liquid chromatography.
    Liu, HL
    Kao, TH
    Chen, BH
    [J]. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2004, 227 : U51 - U51
12345