Determination of theophylline in serum by high performance liquid chromatography mass spectrometry with atmospheric pressure chemical-ionization (APCI HPLC/MS)

被引：7
|
作者：
Susanto, F
Reinauer, H
机构：
[1] Department of Clinical Biochemistry, Diabetes Research Institute, Heinrich-Heine-University, D-40225 Düsseldorf
来源：
FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY | 1997年 / 357卷 / 03期
关键词：
D O I：
10.1007/s002160050164
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A method for the determination of theophylline (TH), without derivatization, in serum by isotope dilution mass spectrometry using labelled [1,3-N-15(2)-2-C-13]theophylline (LTH) as internal standard is described. After deproteinization, the analyte is directly injected into a high performance liquid chromatography-mass spectrometer operating with atmospheric-pressure chemical-ionization (APCI HPLC/MS). The concentrations of TH in sera measured by APCI HPLC!MS are compared with results from gas chromatography - isotope dilution mass spectrometry (GC-ID/MS), high performance liquid chromatography (HPLC) and fluorescence polarization immunoassay (FPIA). The accuracy, precision and recovery of the APCI HPLC/MS and GC-ID/MS methods are discussed. The coefficient of variation (CV) determined from duplicate samples was less than 2%. The detection limit was 10 ng/ml at a signal-to-noise ratio of 3:1.
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页码：338 / 344
页数：7
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