Effects of Leptin on Na+/Ca2+ Exchanger in PC12 Cells

被引：7
|
作者：
Zhang, Zijuan [1 ,3 ]
Sun, Shuguang [1 ]
Du, Caixia [1 ]
Li, Wei [1 ]
Zhang, Juan [1 ]
Zhu, Yanqin [1 ]
Liu, Peilin [2 ]
Xing, Ying [3 ]
机构：
[1] Henan Univ Chinese Med, Basic Med Coll, Expt Teaching Ctr, Zhengzhou, Peoples R China
[2] Zhengzhou Orthopaed Hosp, Dept Spine Surg, Zhengzhou, Peoples R China
[3] Zhengzhou Univ, Dept Physiol, Zhengzhou 450003, Peoples R China
来源：
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | 2016年 / 40卷 / 06期
关键词：
PC12; cells; Calcium; Leptin; Na+/Ca2+-exchanger; OPERATED CA2+ ENTRY; CENTRAL-NERVOUS-SYSTEM; ALZHEIMERS-DISEASE; MESSENGER-RNA; A-BETA; CALCIUM; EXPRESSION; IMMUNOREACTIVITY; ACTIVATION; NEURONS;
D O I：
10.1159/000453203
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Background/Aims: Alzheimer's disease (AD) is known to be related to alterations in neuronal intracellular calcium activity ([Ca2+](i)). The present study revealed the distinct role of leptin in Na+/Ca2+-exchanger activity. Methods: [Ca2+], was determined utilizing Fura-2 fluorescence. The activity of NCX was measured by removal of extracellular Na+ in the presence of external Ca2+. Na+/Ca2+-exchanger activity was further quantified from whole cell currents following removal of extracellular Na+.Na+/Ca2+-exchanger isoform NCX1 transcript levels and protein abundance were quantified by RT-PCR and Western blotting, respectively. Results: Exposure of PC12 cells to 30 mu M amyloid (A beta(42)) increased [Ca2+], an effect significantly blunted by 6 hours incubation with leptin before A beta(42) treatment. Moreover; leptin treatment significantly increased Na+/Ca2+-exchanger mediated Ca+ transport and current, NCX1 transcript level as well as NCX1 membrane protein abundance. Conclusion: We show that leptin blunts A beta(42)-evoked [Ca2+](i), increase by increasing expression and activity of Na+/Ca2+-exchanger NCX1. (C) 2016 The Author(s) Published by S. Karger AG, Basel
引用
收藏
页码：1529 / 1537
页数：9
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