Expression of bioactive recombinant human fibroblast growth factor 9 in oil bodies of Arabidopsis thaliana

被引：15
|
作者：
Yi, Shanyong [1 ,2 ]
Yang, Jing [1 ]
Huang, Jian [1 ,2 ]
Guan, Lili [1 ]
Du, Linna [1 ]
Guo, Yongxin [1 ]
Zhai, Feng [1 ]
Wang, Yanfang [1 ]
Lu, Zhen [1 ]
Wang, Liyong [1 ]
Li, Haiyan [1 ]
Li, Xiaokun [1 ,3 ]
Jiang, Chao [1 ,3 ]
机构：
[1] Jilin Agr Univ, Minist Educ, Engn Res Ctr Bioreactor & Pharmaceut Dev, Changchun 130118, Jilin, Peoples R China
[2] Jilin Agr Univ, Coll Tradit Chinese Med, Changchun 130118, Jilin, Peoples R China
[3] Wenzhou Med Coll, Sch Pharmaceut Sci, Wenzhou 325035, Zhejiang, Peoples R China
来源：
PROTEIN EXPRESSION AND PURIFICATION | 2015年 / 116卷
关键词：
Human fibroblast growth factor 9; Arabidopsis thaliana; Oil body; Fusion protein expression; TRANSGENIC PLANTS; SEEDS; OLEOSINS; SECRETION; PROTEINS; SYSTEMS; FAMILY; SIZE;
D O I：
10.1016/j.pep.2015.08.006
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Fibroblast growth factor 9 (FGF9) has autocrine and paracrine functions in chondrogenesis osteogenesis, hair growth, and gonadal differentiation. We have expressed recombinant human FGF9 (rhFGF9) in the oil bodies of Arabidopsis thaliana via the floral dip method. The expression vector pOTB-rhFGF9 contained an oleosin-rhFGF9 fusion gene and a glufosinate resistance gene for selection. This plasmid was transformed into A. thaliana and expression of the fusion protein oleosin rhFGF9 confirmed by SDS-PAGE and Western blotting. Furthermore, MU assays demonstrated that the oil bodies expressed oleosin rhFGF9 from the transgenic A. thaliana had a remarkable proliferation effect on NIH/3T3 cells. (C) 2015 Published by Elsevier Inc.
引用
收藏
页码：127 / 132
页数：6
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