Genetically modified organisms in food-screening and specific detection by polymerase chain reaction

被引：114
|
作者：
Vollenhofer, S [1 ]
Burg, K [1 ]
Schmidt, J [1 ]
Kroath, H [1 ]
机构：
[1] Austraian Res Ctr Siebersdorf, Biotechnol Unit, A-2444 Seibersdorf, Austria
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1999年 / 47卷 / 12期
关键词：
Polymerase Chain Reaction; PCR; genetically modified organisms; food analysis;
D O I：
10.1021/jf990353l
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
PCR methods for the detection of genetically modified organisms (GMOs) were developed that can be used for screening purposes and for specific detection of glyphosate-tolerant soybean and insect-resistant maize in food. Primers were designed to amplify parts of the 35S promoter derived from Cauliflower Mosaic Virus, the NOS terminator derived from Agrobacterium tunefaciens and the antibiotic marker gene NPTII (neomycin-phosphotransferase II), to allow for general screening of foods. PCR/hybridization protocols were established for the detection of glyphosate-tolerant RoundUp Ready soybean and insect-resistant Bt-maize. Besides hybridization, confirmation of the results using restriction analysis was also possible. The described methods enabled a highly sensitive and specific detection of GMOs and thus provide a useful tool for routine analysis of raw and processed food products.
引用
收藏
页码：5038 / 5043
页数：6
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