Determination of MDMA, MDEA and MDA in urine by high performance liquid chromatography with fluorescence detection

被引：32
|
作者：
da Costa, JL [1 ]
Chasin, AAD
机构：
[1] Univ Sao Paulo, Coll Pharmaceut Sci Toxicol, Instrumental Anal Lab, Criminalist Inst, Av Prof Lineu Prestes,580 B13B Toxicol, BR-05508900 Sao Paulo, Brazil
[2] Coll Oswaldo Cruz, Forens Toxicol Lab, Legal Med Inst, Sao Paulo, Brazil
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2004年 / 811卷 / 01期
关键词：
drug of abuse; MDMA; MDEA;
D O I：
10.1016/j.jchromb.2004.03.076
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This paper describes the development and validation of analytical methodology for the determination of the use of MDMA, MDEA and MDA in urine. After a simple liquid extraction, the analyses were carried out on a high performance liquid chromatography (HPLC) in an octadecyl column, with fluorescence detection. The mobile phase using a sodium dodecyl sulfate ion-pairing reagent allows good separation and efficiency. The method showed good linearity and precision. Recovery was between 85 and 102% and detection limits were 10, 15 and 20 ng/ml for MDA, MDMA and MDEA, respectively. No interfering substances were detected with fluorescence detection. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码：41 / 45
页数：5
相关论文
共 50 条
  • [41] DETERMINATION OF ETHAMBUTOL IN PLASMA BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTION
    Suzuki, Masao
    Ono, Tomoko
    Takitani, Shoji
    ANALYTICAL SCIENCES, 1991, 7 : 949 - 950
  • [42] Determination of Diethylstilbestrol in Aquatic Products by High Performance Liquid Chromatography with Fluorescence Detection
    Xu Yingjiang
    Geng Jinpei
    Zhang Xiuzhen
    Zhang Shijuan
    Tian Xiuhui
    Liu Huihui
    CHINESE JOURNAL OF CHEMISTRY, 2010, 28 (01) : 86 - 90
  • [43] Determination of Aristolochic Acids by High-Performance Liquid Chromatography with Fluorescence Detection
    Wang, Yinan
    Chan, Wan
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2014, 62 (25) : 5859 - 5864
  • [44] Direct screening of urine for MDMA and MDA by liquid chromatography-tandem mass spectrometry
    Nordgren, HK
    Beck, O
    JOURNAL OF ANALYTICAL TOXICOLOGY, 2003, 27 (01) : 15 - 19
  • [45] Determination of ascorbic acid in plasma and urine by high performance liquid chromatography with ultraviolet detection
    Rümelin, A
    Fauth, U
    Halmágyi, M
    CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 1999, 37 (05) : 533 - 536
  • [46] QUANTITATION OF METOLAZONE IN PLASMA AND URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION
    VOSE, CW
    MUIRHEAD, DC
    EVANS, GL
    STEVENS, PM
    BURFORD, SR
    JOURNAL OF CHROMATOGRAPHY, 1981, 222 (02): : 311 - 315
  • [47] Fast LC-MS/MS method for the determination of amphetamine, methamphetamine, MDA, MDMA, MDEA, MBDB and PMA in urine
    Concheiro, Marta
    Sadler Simoes, Susana Maria dos Santos
    Quintela, Oscar
    de Castro, Ana
    Rodrigues Dias, Mario Joao
    Cruz, Angelines
    Lopez-Rivadulla, Manuel
    FORENSIC SCIENCE INTERNATIONAL, 2007, 171 (01) : 44 - 51
  • [48] Determination of terbutaline enantiomers in human urine by coupled achiral-chiral high-performance liquid chromatography with fluorescence detection
    Kim, KH
    Kim, HJ
    Kim, JH
    Shin, SD
    JOURNAL OF CHROMATOGRAPHY B, 2001, 751 (01): : 69 - 77
  • [49] DETERMINATION OF TRIS(HYDROXYMETHYL)AMINOMETHANE (TROMETHAMINE) IN HUMAN PLASMA AND URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION
    MORRIS, MJ
    HSIEH, JYK
    JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1993, 622 (01): : 87 - 92
  • [50] DETERMINATION OF L-3,4-DIHYDROXYPHENYLALANINE IN PLASMA AND URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION
    LEE, M
    NOHTA, H
    OHTSUBO, K
    YOO, B
    OHKURA, Y
    CHEMICAL & PHARMACEUTICAL BULLETIN, 1987, 35 (01) : 235 - 240
12345